Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (50): 9493-.doi: 10.3969/j.issn.1673-8225.2010.50.044

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Progenitor cells in mammalian maxillofacial and mandibular processes
Source and differentiation phenotype

Ze-yuan1, Jin Yan1, Yu Shu-xiang2, Shi Jun-nan1, Zhang Jian-ping3 , Duan Xiao-yan1   

  1. 1 College of Stomatology, the Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shaanxi Province, China; 2 the 97 Hospital of Chinese PLA, Xuzhou  221004, Jiangsu Province, China; 3 School of Life Science and Technology, Xi’an Jiaotong University, Xi’an  710032, Shaanxi Province, China
  • Online:2010-12-10 Published:2010-12-10
  • Supported by:

    the Science Foundation of Shaanxi Province, No. 2008k14-03*

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Abstract:

BACKGROUND: It remains unclear whether ectomesenchymal cells also derived from neural crest stem cell in mammals. 
OBJECTIVE: To understand the specific markers and differentiation directions of maxillofacial and mandibular processes progenitor cells, and to explore the source and differentiation phenotype of ectomesenchymal stem cells.
METHODS: The expression and changes of expression profiles of rat ectomesenchymal cells at E9.5, E10.5, E11.5, and E12.5 days were observed by immunohistochemistry and flow cytometry.
RESULTS AND CONCLUSION: The progenitors expressed multi-lineage markers, including neural system and several mesenchymal tissue types, importantly the facts that molecule profiles were changed with time prolonged, suggesting these progenitors were in active differentiating stage, so they were stem like cells or contain stem like cells. Moreover, small populations (2%-3%) of CD57 and P75 phenotypes were detected by flow cytometry, suggesting that ectomesenchymal stem cells were derived from neural crest, which maintained a quantitative stabilization though it is gradually differentiate after localization.

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