Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (50): 9311-9316.

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Expression of programmed cell death 5 in degenerated lumbar intervertebral disc

Cui Guan-yu1, Tian Wei1, Zhao Dan-hui2, Liu Bo1, Li Guang-ping2, Chen Ying-yu3   

  1. 1 Department of Spinal Surgery, Beijing Jishuitan Hospital, Beijing  100035, China; 2 Beijing Research Institute of Traumatology and Orthopaedics, Beijing  100035, China; 3 Center for Human Disease Genomics, Health Science Center, Peking University, Beijing  100191, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Tian Wei, Professor, Chief physician, Doctoral supervisor, Department of Spinal Surgery, Beijing Jishuitan Hospital, Beijing 100035, China
  • About author:Cui Guan-yu★, Master, Physician, Department of Spinal Surgery, Beijing Jishuitan Hospital, Beijing 100035, China gycui98@yahoo.com.cn
  • Supported by:

    the General Program of the National Natural Science Foundation of China, No. 30571752*, 30470844*

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Abstract:

BACKGROUND: Cell apoptosis plays an important role in intervertebral disc degeneration. Programmed cell death 5 (PDCD5) is a protein which promotes cell apoptosis and upgrade expressed in osteoarthritic cartilage. But the study of expression of PDCD5 on degenerated lumbar intervertebral disc has not been reported until now.
OBJECTIVE: To investigate the expression of PDCD5 in nucleus pulposus cells from normal, protruded and extruded lumbar intervertebral disc and to analyze the relationship between PDCD5 and lumbar intervertebral disc degeneration.
METHODS: Samples from 2 normal, 23 protruded and 17 extruded lumbar intervertebral discs were collected. The expression of PDCD5 was detected with immunohistochemical staining and immunofluorescence detection. Nucleus pulposus cell apoptosis was identified by TUNEL and transmission electronic microscope. The extracellular matrix degeneration was detected with sirius red staining.
RESULTS AND CONCLUSION: PDCD5 positive rate of nucleus pulposus cells from extruded intervertebral disc was higher than that from protruded intervertebral disc (P < 0.05). TUNEL detection positive rate of nucleus pulposus cells from extruded intervertebral disc was higher than that from protruded intervertebral disc (P < 0.05). PDCD5 was observed to be located in cell nucleus with confocal laser scanning microscopy. Cell apoptosis can be observed with transmission electronic microscope. With age increased, type Ⅰ collagen increased and typeⅡcollagen decreased in nucleus pulposus. In degenerated intervertebral disc, the expression of PDCD5 and nucleus pulposus cells apoptosis increased with age increased and disc degeneration scale increased. Nucleus pulposus cells apoptosis through PDCD5 pathway plays an important role in lumbar intervertebral disc degeneration.

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