Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (33): 6184-6188.

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Relationship of synovium interleukin-1, interleukin-6 and leukotriene level changes to injection timing in rabbits with papain-induced knee osteoarthritis

Sun Lu-ning1, Huang Gui-cheng2, Zhao Yan-hua2, Xia Jian-long2   

  1. 1The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China;
    2Nanjing University of Chinese Medicine, Nanjing 210029, China
  • Received:2012-02-24 Revised:2012-04-20 Online:2012-08-12 Published:2012-08-12
  • Contact: Huang Gui-cheng, Master, Professor, Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • About author:Sun Lu-ning☆, Studying for doctorate, Associate chief physician, the Affiliatet Hospital of Nanjing University of Chinese Medicine, Nanjing 210029 , Jiangsu Province, China sunluning2000@ sina.com

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Abstract:

BACKGROUND: A stable degenerative knee osteroarthritis model in rabbits can be established by the induction of papain.
OBJECTIVE: To ivestigate the relationships of the level changes of synovium interleukin-1 interleukin-6 and leukotriene to injection time in rabbits with knee osteoarthritis induced by intraarticular injection of papain.
METHODS: Totally 18 New Zealand male white rabbits were divided into 2 weeks group, 4 weeks group and 6 weeks group randomly (6 rabbits in each group). Papain solution was injected into the right knee joint cavity of each rabbits at days 1, 4 and 7, and rabbit model of knee osteoarthritis was established. The rabbits of corresponding groups were killed at weeks 2, 4 and 6 after first injection. And then, interleukin-1, interleukin-6 and leukotriene concentrations of right knee synovium were examined. Normal rabbit left knee synovium of the 2 weeks group were examined simultaneously as the control group.
RESULTS AND CONCLUSION: Level of interleukin-1, interleukin-6 and leukotriene at week 2 was higher than those of the control group. There was significant difference between the former two groups (P < 0.05). In terms of the levels of interleukin-1, interleukin-6 and leukotriene, there was no significant difference between the 4 week and 2 week groups (P > 0.05). There was no significant difference between the 6 week group and the control group in the level of interleukin-1 and interleukin-6 (P > 0.05). The leukotriene of the week 6 group was higher than that of the control group (P < 0.05), and was lower than that of the 4 week group (P < 0.05). These results suggest that the level changes of synovial inflammatory factors in rabbit knee joint are significantly increased within 4 weeks after the first injection of papain.