Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (33): 6087-6090.doi: 10.3969/j.issn.2095-4344.2012.33.002

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Perlecan antibody can inhibit synthesis and secretion of transforming growth factor beta 1 in osteoblasts cultured in vitro

Tian Zhao-yan, Ye Fa-gang, Chu Yan-chen   

  1. Department of Traumatology, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China
  • Received:2011-11-09 Revised:2012-01-11 Online:2012-08-12 Published:2012-08-12
  • Contact: Ye Fa-gang, Doctor, Professor, Master’s supervisor, Department of Traumatology, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China Yfg2008@yeah.net
  • About author:田召彦★，男，1983出生，山东省淄博市人，汉族，青岛大学在读硕士，主要从事骨外科专业方面的研究。 82338965@qq.com

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Abstract:

BACKGROUND: Up to now, there are few reports about the action mechanism of perlecan underlying fracture healing as well as effects of perlecan on proliferation of osteoblasts by regulation of transforming growth factor beta 1 (TGFβ1).
OBJECTIVE: To investigate the effect of perlecan on synthesis and secretion of TGF-β1 in osteoblasts from the calvaria of fetal rats cultured in vitro.
METHODS: Osteoblast-like cells were isolated from the calvaria of SD fetal rats and cultured. The cultured cells were identified using alkaline phosphatase staining and alizarin red staining. Then, the cells were divided into control and perlecan block groups. The synthesis and secretion of TGF-β1 was examined by using immunohistochemical method and enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The level of TGFβ1 was significantly lower in the perlecan block group than the control group (P < 0.05). Perlecan antibody could inhibit the synthesis and secretion of TGFβ1 in osteoblasts cultured in vitro.

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