Abstract:

BACKGROUND: Early studies have shown that RT-Q medical biomembrane has a significant hemostatic effect on topical bleeding, and possesses good histocompatibility.
OBJECTIVE: To investigate the main biocompatibility indices of RT-Q medical biomembrane.
METHODS: First, MTT method was used to determine the cytotoxicity of 0 (control), 12.5%, 25%, 50% and 100% RT-Q medical biomembrane on L2929 cells. Second, 16 rabbits were randomly divided into negative control group, positive control group, intact skin group and broken skin group. Rabbits in the former three groups were sprayed with normal saline, formaldehyde and RT-Q medical biomembrane, respectively, on the right side of the spine skin. While, rabbits in the broken skin group were only sprayed with RT-Q medical biomembrane on the right side of the spine skin after piercing in the bilateral spine skin. Next, the 12 rabbits were sprayed with RT-Q medical biomembrane and injected with normal saline after piercing the vain of rabbit’s ear border. Last, 30 Guinea pigs were sprayed with RT-Q medical biomembrane, normal saline and 2,4-dinitrochlorobenzene, respectively.
RESULTS AND CONCLUSION: In cytotoxicity test, various concentrations of RT-Q medical biomembrane showed non-toxicity to L2929 cells. Besides, RT-Q medical biomembrane was nonirritant to the intact and broken skin of animals as well as broken vessels. In addition, there were no allergic reactions and it had the hemostatic effect. These results suggest that RT-Q medical biomembrane have passed the main tests of biocompatibility for its good biocompatibility.