Casein phosphopeptides/amorphous calcium phosphate compound and fluoride prevent enamel demineralization around orthodontic brackets

doi:10.3969/j.issn.2095-4344.2016.03.013

Abstract

Abstract:

BACKGROUND: In clinical practice, both casein phosphopeptides/amorphous calcium phosphate compound and fluoride can effectively prevent the occurrence of enamel demineralization and caries.
OBJECTIVE: To investigate the preventive effects of casein phosphopeptides/amorphous calcium phosphate compound and fluoride on enamel demineralization in the orthodontic treatment, so as to seek a remineralization agent with good effect.
METHODS: Totally 40 human in vitro teeth which had been well bonded to brackets were randomly divided into four groups: control group (the surface was not coated with any substance), GC tooth mousse group (the surface was coated with casein phosphopeptides/amorphous calcium phosphate compound), duraphat group (the surface was coated with fluoride) and combined group (the surface was coated with casein phosphopeptides /amorphous calcium phosphate compound and fluoride). The enamel surface porosity was observed by scanning electron microscopy after the successive 14-day demineralization-remineralization experiments in these four groups. The change value of microhardness was measured before and after the experiment in each group.
RESULTS AND CONCLUSION: There was obvious enamel demineralization in the control group, but not in the other three groups. The change value of microhardness in GC tooth mousse, duraphat and combined groups was lower than that in the control group (P < 0.05), and there was no significant difference between GC tooth mousse, duraphat and combined groups. These results demonstrate that fluoride, casein phosphopeptides/amorphous calcium phosphate compound and their combination can prevent enamel demineralization, promote remineralization; however, their combination does not improve the effect of preventing enamel demineralization.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Luo Hui, Jia De-jiao, Liu Hai-xia, Fan Ya-ru, Zhao Hao-ran . Casein phosphopeptides/amorphous calcium phosphate compound and fluoride prevent enamel demineralization around orthodontic brackets[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(3): 377-381.

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References

[1] 招顺秀,李业荣,许碧晖.多乐氟预防固定正畸矫治中牙釉质脱矿的临床研究[J].广东牙病防治,2013,21(8):441-443.

[2] Nalbantgil D,Oztoprak MO,Cakan DG,et al.Prevention of demineralization around orthodontic brackets using tow different fluoride varnishes.Eur J Dent.2013;7(1):41-47.

[3] Pol TR,Naik CR,Gupta R,et al.Incorporation of casein phosphopeptide-amorphous calcium phosphate into glass-ionomer cement for orthodontic band cementation-an in vitro study.Int J Stomatol Res.2013;2:5-10.

[4] Tüfekçi E,Pennella DR,Mitchell JC,et al.Efficacy of a fluoride-releasing orthodontic primer in reducing demineralization around brackets: an in-vivo study. Am J Orthod Dentofacial Orthop.2014;146(2):207-214.

[5] Yap J,Walsh LJ,Naser-Ud Din S,et al.Evaluation of a novel approach in the prevention of white spot lesions around orthodontic brackets.Aust Dent J.2014;59(1):70-80.

[6] Ballard RW,Hagan JL,Phaup AN,et al.Evaluation of 3 commercially available materials for resolution of white spot lesions.Am J Orthod Dentofacial Orthop.2013;143(4 Suppl): S78-84.

[7] Zanarini M,Pazzi E,Bonetti S,et al.In vitro evaluation of the effects of a fluoride-releasing composite on enamel demineralization around brackets.Prog Orthod. 2012; 13(1):10-16.

[8] Lodaya SD,Keluskar KM,Naik V.Evaluation of demineralization adjacent to orthodontic bracket and bond strength using fluoride-releasing and conventional bonding agents.Indian J Dent Res.2011;22(1):44-49.

[9] Chow CK,Wu CD,Evans CA.In vitro properties of orthodontic adhesives with fluoride or amorphous calcium phosphate.Int Dent.2011;2011:583521.

[10] 刘兴容,徐皑,李艳萍.酷蛋白磷酸肽钙磷复合体对变形链球菌生长、产酸的影响[J].现代预防医学,2006,33(10):1815-1817.

[11] 方萍,陈燕,周嫣,等.护牙素对正畸牙面脱矿修复的临床效果观察[J].广西医学,2012,34(8):978-980.

[12] Hu W,Feath erstone JD.Prevention of enamel demineralization:an in-vitro study using light-cured ?lled sealant. Am J Othod Dentofacial Orthop.2005;128:592-600.

[13] 胜华,高珊,王海燕.氟保护漆对正畸牙釉质脱矿影响的研究[J].口腔医学,2013,33(3):155-157．

[14] 张晓慧,刘梅天,冯致远,等.氟化物在正畸釉质粘接剂中的应用[J].中国组织工程研究与临床康复,2011,15(21):3925-3927.

[15] 景先明,冯云霞,李罡.酪蛋白磷酸肽抑制人牙釉质脱矿作用的体外实验研究[J].中国实用口腔科杂志,2009,2(8):472-473．

[16] 韩红娟,吴浩,任小华.多乐氟治疗牙齿正畸白斑的效果观察[J].西部医学,2013,25(11):1632-1633.

[17] Uysal T,Amasyali M,Koyuturk AE,et al.Effects of different topical agents on enamel demineralization around orthodontic brackets: an in vivo and in vitro study. Aust Dent J. 2010;55(3): 268-274.

[18] 钟春梅,刘进,葛元输,等.接受固定正畸治疗的患者口腔卫生状况追踪观察[J].临床口腔医学杂志,2010,26(5):290-292.

[19] 朱吉坤,张桂荣,郭艳明,等.儿童正畸矫治中多乐氟预防牙齿脱矿的功效探讨[J].国际儿科学杂志,2014,41(2):202-204.

[20] 米君国,吴丽萍.正畸治疗中氟化物抑制牙釉质脱矿的体外研究[J].同济大学学报, 2012,33(3):37-41.

[21] 陈晨,钟圣纯.氟保护漆预防龋齿的进展[J].中国现代医药杂志, 2009,12(11):129-131.

[22] Eevarathan J,Deepti A,Muthu MS,et al.Effect of fluoride varnish on Streptococcus mutans counts in plaque of caries-free children using Dentocult SM strip mutans test: a randomized controlled triple blind study. J Indian Soc Pedod Prev Dent.2007; 25(4): 157-163.

[23] 阳宏林,李炯,杨梓.氟保护漆预防正畸治疗中釉质脱矿的临床研究[J].安徽医科大学学报, 2011,46(6):588-589.

[24] Oshiro M,Yamaguchi K,Takamizawa T,et al.Effect of CPP-ACP paste on tooth mineralization: an FE-SEM study. Oral Sci.2007;49(2):115-120.

[25] Rahiotis C,Vougiouklakis G.Effect of a CPP-ACP agent on the demineralization and remineralization of dentine in vitro. J Dent.2007;35(8):695-698.

[26] 李淑慧,吴佩玲.酪蛋白磷酸多肽-无定形磷酸钙在口腔治疗中的研究应用[J].牙体牙髓牙周病学杂志,2011,21(10):601-603.

[27] Srinivasan N,Kavitha M,Loganathan SC.Comparison of the remineralization potential of CPP-ACP and CPP-ACP with 900ppm fluoride on eroded human enamel：an in situ study. Arch Oral Biol.2010;55(7):541-544.

[28] Erdem AP,Sepet E,Avshalom T,et al.Effect of CPP-ACP and APF on Streptococcus mutans biofilm: a laboratory study.Am J Dent.2011;24(2):119-123.

[29] Rose RK. Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques. Arch Oral Biol.2000;45(7):569-575.

[30] 鲍玮玮.护牙素和氟化物预防正畸牙面脱矿的体外研究[D].河北:河北医科大学学位论文, 2012:8-11.

[31] Takenloto S,Hatori M,Yoshinori M,et al.Corrosion behavior and surface characterization of titanium in solution containing fluoride and albumin. Biomaterials. 2005;26(8):829-837.