Action mechanisms of active vitamin D3 on protecting the liver of type 2 diabetes mellitus rats

doi:10.3969/j.issn.2095-4344.2016.05.015

Abstract

Abstract:

BACKGROUND: Active vitamin D3 plays an important role in the development of type 2 diabetes and its complications.
OBJECTIVE: To explore the influence of active vitamin D3 on the liver of type 2 diabetes mellitus rats, and its mechanisms.
METHODS: A total of 35 male Sprague-Dawley rats were randomly divided into normal control group, diabetes group and vitamin D3 group. In the diabetes group and vitamin D3 group, rats received high fat and high sugar diet and intraperitoneal injection of streptozotocin to prepare rat models of diabetes mellitus. In the vitamin D3 group, rats were intragastrically given calcitriol dissolved in peanut oil 0.03 μg/kg per day. In the normal control group and diabetes group, rats were intragastrically given peanut oil. 8 weeks later, rats were sacrificed and serum was isolated. Fasting blood glucose, fasting insulin, total cholesterol and triacylglycerol levels were measured. Insulin resistance index in the steady-state model was calculated. The liver was retained, and subjected to hematoxylin-eosin staining, immunohistochemical staining, and real-time fluorescent quantitative PCR.
RESULTS AND CONCLUSION: (1) Compared with the diabetes group, triacylglycerol and insulin resistance index were lower in the vitamin D3 group (P < 0.05). (2) Compared with the normal control group, swelling of the liver cells and fatty degeneration with inflammatory cell infiltration were found. Protein expression of JNK and C-Jun and phosphorylation levels, mRNA expression of JNK and C-Jun, tumor necrosis factor α and interleukin-1β increased in the diabetes group (P < 0.05). Liver cell swelling and fatty degeneration lessened, inflammatory cell infiltration reduced in the vitamin D3 group. Simultaneously, the expression of above factors was lower in the vitamin D3 group than in the diabetes group (P < 0.05). (3) Results suggested that the protective effect of vitamin D3 on the liver of rats with type 2 diabetes was possibly associated with its effect on downregulating JNK/C-Jun signaling pathway.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Liu Li-na, Wang Zhi-qiang, Zhu Jun. Action mechanisms of active vitamin D3 on protecting the liver of type 2 diabetes mellitus rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(5): 694-700.

Figures/Tables 3

References

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