Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (52): 9787-9791.doi: 10.3969/j.issn.2095-4344.2012.52.019

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Changes of insulin-like growth factor 1 levels in rabbits with steroid-induced femoral head necrosis after treatment with Huoxuetongluo method

Zhang Chi1, Yang Zhi-wei2, Duan Xiao-ying1   

  1. 1Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China
    2Ya'an Vocational College, Ya’an 625000, Sichuan Province, China
  • Received:2012-05-04 Revised:2012-10-08 Online:2012-12-23 Published:2012-12-23
  • Contact: Yang Zhi-wei, Lecturer, Ya'an Vocational College, Ya’an 625000, Sichuan Province, China yayzw@126.com
  • About author:Zhang Chi★, Master, Associate professor, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China zhangchid@126.com

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Abstract:

BACKGROUND: Huoxuetongluo method is an effective prescription for the treatment of steroid induced femoral head necrosis in clinic, and Huoxuetongluo can promote osteonecrosis tissue repair.
OBJECTIVE: To investigate the effect of Huoxuetongluo method on the level of insulin-like growth factor-1 in the steroid induced femoral head necrosis rabbit model.
METHODS: The rabbits were randomly divided into five groups, and the rabbits in four groups were used to establish the steroid induced femoral head necrosis model through injecting the glucocorticoid. The rabbits in the high, moderate and low dose groups were administered intramuscularly with 10.0, 5.0 and 2.5 mL/kg Huoxuetongluo decoction, rabbits in both the model group and the blank control group were administered intramuscularly with 10 mL/kg normal saline and lasted for 8 weeks.
RESULTS AND CONCLUSION: Histomorphometric examination showed that the femoral head necrosis in the model group was severe, while the femoral head necrosis in the high, moderate and low dose groups was mild necrosis. The data of empty lacuna in the model group was significantly higher than that in the blank control group (P < 0.01), and the level in the high, moderate and low dose groups was lower than that in the model group (P < 0.05); the level of insulin-like growth factor-1 in the model group was higher than the in the blank control group (P < 0.05) and the level in the high, moderate and low dose groups was higher than that in the model group (P < 0.01), while there was no significant difference between the every two Huoxuetongluo groups (P > 0.05). Huoxuetongluo decoction can promote the repairing and reconstruction of femoral head necrosis through increasing the level of insulin-like growth factor-1, but the level of insulin-like growth factor-1 will not increased with the increasing of the dose of Huoxuetongluo decoction when the dose reached to a certain level.

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