Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1123-1126.doi: 10.3969/j.issn.1673-8225.2011.06.039

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Simulation of stem cell microenvironment in vitro

Qiu Yun, Zheng Qing, Xiao Shu-dong, Wang Zheng   

  1. Department of Digestion, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Diseases, MED-X Renji Stem Cell Clinical Research Center, Shanghai Jiao Tong University, Shanghai  200001, China
  • Received:2010-09-14 Revised:2010-11-08 Online:2011-02-05 Published:2011-02-05
  • Contact: Zheng Qing, Doctor, Associate professor, Department of Digestion, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Diseases, MED-X Renji Stem Cell Clinical Research Center, Shanghai Jiao Tong University, Shanghai 200001, China qingzheng101@yahoo.com Wang Zheng, Doctor, Associate professor, Department of Digestion, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Diseases, MED-X Renji Stem Cell Clinical Research Center, Shanghai Jiao Tong University, Shanghai 200001, China zheng.w.dr@gmail.com
  • About author:Qiu Yun★, Studying for master’s degree, Department of Digestion, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Diseases, MED-X Renji Stem Cell Clinical Research Center, Shanghai Jiao Tong University, Shanghai 200001, China qyun200911@yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No.30971468*

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Abstract:

BACKGROUND: Extracellular matrix (ECM) is an important component of the cellular niche in a tissue, supplying critical biochemical and physical signals to sustain cellular functions.
OBJECTIVE: To review the research progress of in vitro simulation of bone marrow stem cell microenvironment, and to introduce reconstruction of ECM in vitro.
METHODS: The database of Pubmed (1999-01/2010-10) and Embase (1999-01/2010-10) was utilized to search the related articles about stem cell microenvironment. Key words were “mesenchymal stem cells, extracellular matrix, niche, growth factor modulation, culture in vitro”. There were 321 articles after the initial survey. A total of 23 articles related to stem cell microenvironment were selected according to inclusion criteria.
RESULTS AND CONCLUSION: The established bone marrow-derived ECM culture system retains mesenchymal stem cell properties and may facilitate efforts to purify these cells and to produce a large number of relatively homogenous mesenchymal stem cells. Establishment of a unique scaffold built by 3D synthetic materials coated with tissue-specific ECM proteins and deposited with an appropriate combination of growth factors will facilitate the control of the differentiation direction of mesenchymal stem cells.

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