Abstract:

BACKGROUND: Previous studies have demonstrated that salvia miltiorrhiza can decrease inducible nitric oxide synthase (iNOS) mRNA expression, reduce nitrogen monoxidum production, inhabit tumor necrosis factor and interleukin secretion, as well as antioxidation. As a monomer component of salvia miltiorrhiza, whether Dasensu has the similar effects remain poorly understood.
OBJECTIVE: To verify the intervention of Danshensu on heme oxygenase-1 (HO-1) expression protection on liver cold preservation in vitro.
METHODS: Rats were prepared for models of liver hypothermic perfusion preservation and divided into 3 groups. Rats in the control group were intraperitoneal injected physiological saline preoperatively and infused by Ringer lactate solution; those in the experimental group were received intraperitoneal injection of physiological saline preoperatively and infused by Danshensu plus infused by Ringer lactate solution in the surgery; those in the inhibitor group were intraperitoneal injected original zinc porphyrin preoperatively and infused by Danshensu plus infused by Ringer lactate solution in the surgery. HO-1mRNA and protein expression was determined by RT-PCR; Mitochondrial calcium content and calcium ions ATP activity were detected, and the hepatocyte and mitochondrial morphological changes were observed under a electron microscopy, and the morphological changes of liver cells and lobular were observed under a light microscope.
RESULTS AND CONCLUSION: Hepatic HO-1 mRNA and protein levels of the experimental group were significantly higher than that of the other groups (P < 0.05). Compared with the other groups, the liver mitochondrial calcium content was notably lower in the experimental group, but the Ca2+-ATP activity was greater. The results reveled that, Danshensu perfusion fluid can induce over-expression of HO-1 and prolong the liver cold preservation time.