Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (47): 8759-8763.doi: 10.3969/j.issn.1673-8225.2010.47.005

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Calcium hydrogen phosphate/collagen composite versus hydroxyapatite/collagen composite for repair of massive bone defects

Hu Qing-liu 1,2   

  1. 1 Nanjing Medicine Science & Technology Co., Ltd., Nanjing   210061, Jiangsu Province, China; 2 Research Institute of Tsinghua University, Shenzhen   518057, Guangdong Province, China
  • Online:2010-11-19 Published:2010-11-19
  • About author:Hu Qing-liu☆, Doctor, Investigator, Nanjing Medicine Science & Technology Co., Ltd., Nanjing 210061, Jiangsu Province, China; Research Institute of Tsinghua University, Shenzhen 518057, Guangdong Province, China huqingliuth@yahoo.com.cn
  • Supported by:

    the National High-Tech Small and Middle Enterprises Innovation Fund, No. 09C26213213809*; Science and Technology Projects in Nanjing, No. 200904003*; Shenzhen Basic Research Projects, No. JC200903170487A*; Science and Technology Projects in Nanshan District of Shenzhen, No. 2008018*

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Abstract:

BACKGROUND: Hydroxyapatite or the complex with other components has become the international standard materials for bone repair, which has been widely used in clinical and experimental research. Their biocompatibility has been fully verified, but poor performance of degradability and limited ability to induce bone regeneration are also recognized. We believe that hydroxyapatite as a bone induction material is only to mechanically mimic the composition of natural bone, other calcium phosphate salts as inorganic components of bone composites may lead to breakthrough the fixed current scientific thinking and provide a new aspect of artificial bone development.
OBJECTIVE: To compare the osteo-conductivity and osteo-inductivity of calcium hydrogen phosphate/collagen (DCP/CO) composite with hydroxyapatite/collagen (HA/CO) composite.
METHODS: Fifteen New Zealand rabbits were used in this experiment to prepare a 10-mm bone defect at left ulnas, and then implanted with DCP/CO composite, standard HA/CO composite and synthesized HA/CO composites. The rabbits were killed at 92 postoperative days after the completion of X-ray photography, and processed into anatomical observation and histological examination.
RESULTS AND CONCLUSION: X-ray photography showed that, the defects were completely healed owing to bone hyperblastosis in the DCP/CO composite group at 92 days after surgery, anatomical observation demonstrated that the repaired defects were similar to the appearance of non-surgical site. Histological sections showed the bone lamella formed at the defect site and fully ossified, with obvious osteon, there was no remarkable layered bone lamella, osteocytes were visible in the scattered absconsiones, and new vessels in central canal, without artificial bone residue. The artificial bone was completely biodegraded and absorbed. At 92 days after surgery, the defects of ulnas in standard HA/CO group were broken easily, indicating poor ossification; in HA/CO group the defect remained unrpaired. DCP/CO composite (imitating cancellous bone bioactive bone), produced by Nanjing Medicine Science & Technology Co., Ltd., has a superior osteo-conductivity and osteo-inductivity than HA/CO composite.

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