Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7791-7794.doi: 10.3969/j.issn.1673-8225.2010.42.001

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Hydroxyapatite/tricalcium phosphate combined with bone morphogenetic protein and basic fibroblast growth factor repairs bone defects in rabbits

Zheng Jun, Tang Wei-zhong, Qi Xin-sheng   

  1. Department of Orthopaedic Surgery, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai   200437, China
  • Online:2010-10-15 Published:2010-10-15
  • About author:Zheng Jun★, Master, Attending physician, Department of Orthopaedic Surgery, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China Dudu8238@sina.com
  • Supported by:

    Jiangsu Provincial Science and Technology Commission, No. BG2001033*

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Abstract:

BACKGROUND: Cytokines can initialize, promote and maintain cartilage and bone formation, but require a suitable carrier to exert biological activity.
OBJECTIVE: To investigate the repaired effects of bone defected by the compound of hydroxyapatite/tricalcium phosphate (1:1, HA/TCP) combined with bone morphogenetic protein (BMP) and basic fibroblast growth factor (bFGF).
METHODS: A 4 mm × 4 mm × 12 mm bone defect was produced in both thighbone of 27 experimental rabbits, which were randomly divided into three groups, BMP + bFGF + HA/TCP, BMP + HA/TCP, HA/TCP groups. The repair of bone defects in each group was investigated by means of hematoxylin-eosin staining, X-ray examination, and computer image analysis at 4, 8, 12 weeks after operation.
RESULTS AND CONCLUSION: BMP + bFGF + HA/TCP group and BMP + HA/TCP group were superior to HA/TCP group regarding the cartilage induction, trabecular bone formation and bone defects repair. In addition, BMP + bFGF + HA/TCP group was better than BMP + HA/TCP group. At 8 weeks after operation, woven bone trabecular gradually developed into mature lamellar bone and pulp cavity, bone defects were almost repaired. At 12 weeks, bone defects were completely repaired. Results indicated that the compound of HA/TCP, BMP and bFGF possesses strong osteogenesis potential and the ability of rebuilding bone defect. BMP combined with bFGF can apparently promote new bone formation. The porous HA/TCP is a good controlled-release scaffold material and can absorb cytokines, its degradation and absorption are interacted with new bone formation.

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