Granule cartilage plus n-butyl α-cyanoacrylate glue combined with 3D printing technology for preparation of individualized ear scaffolds

doi:10.3969/j.issn.2095-4344.2321

Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (34): 5520-5525.doi: 10.3969/j.issn.2095-4344.2321

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Granule cartilage plus n-butyl α-cyanoacrylate glue combined with 3D printing technology for preparation of individualized ear scaffolds

Zhang Fan¹, Li Gaofeng², Hu Yigao², Cai Lihong³

¹Xiangtan Aist Medical Beauty Clinic, Xiangtan 411100, Hunan Province, China; ²Department of Plastic Laser Cosmetology, Hunan Provincial People’s Hospital (the First Affiliated Hospital of Hunan Normal University), Changsha 410005, Hunan Province, China; ³Department of Radiology, The Third Hospital of Changsha, Changsha 410007, Hunan Province, China

Received:2019-12-12 Revised:2019-12-14 Accepted:2020-01-18 Online:2020-11-08 Published:2020-09-11
Contact: Li Gaofeng, MD, Chief physician, Professor, Master’s supervisor, Department of Plastic Laser Cosmetology, Hunan Provincial People’s Hospital (the First Affiliated Hospital of Hunan Normal University), Changsha 410005, Hunan Province, China
About author:Zhang Fan, Master, Physician, Xiangtan Aist Medical Beauty Clinic, Xiangtan 411100, Hunan Province, China
Supported by:
Scientific Research Project of Hunan Provincial Department of Education (General Program), No. 16C0964

Abstract

Abstract:

BACKGROUND: The manufacture of ear brackets in traditional ear reconstruction is likely to cause ear distortion, and 3D printing technology makes it possible to prepare individualized and highly simulated ears.

OBJECTIVE: To explore the feasibility of making individualized ear scaffolds using granule cartilage plus n-butyl α-cyanoacrylate glue with 3D printing technology.

METHODS: Male and female modes of 3D ear scaffolds were prepared for the affected ears according to the ears on the non-injured side. Granule cartilage and n-butyl α-cyanoacrylate glue were placed in the female modes to prepare ear cartilage scaffolds through slight adjustment based on male modes. Then the ear cartilage scaffolds were implanted into the back of New Zealand rabbits. The morphology of constructed ear scaffolds was dynamically observed. At postoperative 4, 8, and 12 weeks, the ear scaffolds were taken out in batches. The connection between granule cartilage and cartilage survival was histologically observed. Immunohistochemistry was performed to detect the expression of type II collagen. The animal experiments were approved by the Animal Ethics Committee of Hunan Normal University, China (approval No. 201517).

RESULTS and CONCLUSION: (1) Gross observation: compared with before surgery, the length, width and height of the reconstructed ears were not significantly changed, but the weight of the reconstructed ears was significantly increased at 4, 8, and 12 weeks after surgery (P < 0.05). (2) Histological observation: Inflammatory cell infiltration was observed around granular cartilage. With the prolongation of ear scaffold implantation time, inflammatory cell count gradually decreased, and there were more newly formed vessels in the fascia attached to the scaffold. With the prolongation of implantation time, the number of new chondrocytes in the cartilage membrane gradually increased, cartilage volume gradually increased, and mature chondrocytes gradually increased. Obvious decomposition of n-butyl α-cyanoacrylate between cartilage pieces was not observed. Most of the cartilage pieces were still separated by n-butyl α-cyanoacrylate, and a few of cartilage pieces had fiber connections. With the prolongation of implantation time, the connections between cartilage pieces increased. (3) Immunohistochemistry observation: With the prolongation of ear scaffold implantation time, the expression of type II collagen gradually increased. (4) These results suggest that granule cartilage plus n-butyl α-cyanoacrylate glue combined with 3D printing technology make preparation of individualized ear scaffolds feasible.

Key words:

material, granule cartilage, n-butyl α-cyanoacrylate, ear scaffold, individualized, auricle reconstruction, 3D printing

CLC Number:

Zhang Fan, Li Gaofeng, Hu Yigao, Cai Lihong. Granule cartilage plus n-butyl α-cyanoacrylate glue combined with 3D printing technology for preparation of individualized ear scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(34): 5520-5525.

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Granule cartilage plus n-butyl α-cyanoacrylate glue combined with 3D printing technology for preparation of individualized ear scaffolds

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