Application of cell sheet technology in tissue engineering

doi:10.3969/j.issn.2095-4344.2016.11.017

Abstract

Abstract:

BACKGROUND: The cell sheet technology that is applied with the absence of scaffolds and enzymatic digestion can effectively repair tissue defects and improve organ function, by stimulating the secretion of extracellular matrix to form a dense membrane tissue.
OBJECTIVE: To review the recent progress in cell sheet technology used in tissue engineering, thereby providing a new idea for relevant basic and clinical research.
METHODS: The first author retrieved CNKI database, Wanfang database and PubMed with the keywords of “cell sheet, tissue engineering” in Chinese and English, respectively. Literature retrieval period was from January 2010 to July 2015.
RESULTS AND CONCLUSION: Cell sheet technology combined with scaffold materials can be used for reconstruction and repair of tissues and organs. With the emerging of new technologies, multi-layer cell sheets are stratified to form a three-dimensional tissue for repair of soft tissues and organs. Compared with the monolayer cell sheet, the three-dimensional cell sheet that is laminated by same or different cell sheets has stronger regenerative ability and can be used to construct the ideal target tissue model in vitro. Cell sheet technology combined with scaffolds can improve the mechanical strength of the composite and reduce cell loss, which has made great progress in the repair of tooth, bone and cartilage tissue. Currently, the cell sheet technology is at the laboratory stage, and little is reported on its clinical applications. We look forward to more innovative technologies that can be integrated into the cell sheet technology.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Zhou Shu-kui, Zhang Kai-le, Wang Ying, Fu Qiang. Application of cell sheet technology in tissue engineering[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(11): 1630-1636.

Figures/Tables 1

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