Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (11): 1558-1583.doi: 10.3969/j.issn.2095-4344.2016.11.006

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Expression of Livin, Smac and Caspase-3 in keloids and their correlation

Zhang Yuan-gui1, Duan Dong1, Li Pan-deng1, Chen Run-fang2, Li Zhi-hua1, Gao Xin-yu1, Wu Wei1
  

  1. 1Department of Burns and Plastic Surgery, 2Department of Laboratory Medicine, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
  • Received:2016-01-04 Online:2016-03-11 Published:2016-03-11
  • About author:Zhang Yuan-gui, Master, Chief physician, Department of Burns and Plastic Surgery, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
  • Supported by:

    the Social Development-Clinical Medicine Research Project of Xuzhou Science and Technology Department, No. XM13B088

Abstract:

BACKGROUND: Currently, there is no effective treatment for keloids that often recur. Its pathogenesis is still entirely unclear, and fibroblast proliferation and apoptosis have become a research hotspot.
OBJECTIVE: To investigate the expression of Livin, Smac and Caspase-3 in keloids and to analyze their relationship so as to preliminarily explore the significance of Livin, Smac and Caspase-3 in the pathogenesis of keloids.
METHODS: RT-PCR and immunohistochemical methods were used to detect the mRNA and protein expressions of Livin, Smac and Caspase-3 in keloids (n=20) and normal skin tissues (n=20).
RESULTS AND CONCLUSION: Compared with the normal skin tissue, the mRNA and protein positive expressions of Livin were significantly higher in keloids (P < 0.05), while the mRNA and protein positive expressions of Smac and Caspase-3 were lower in keloids (P < 0.05). There was a negative association between Livin and Smac, Caspase-3 protein expression in keloids. These findings indicate that the high mRNA expression of Livin may cause the imbalance between proliferation and apoptosis of fibroblasts by inhibiting the mRNA expression of Smac and Caspase-3, and eventually lead to the formation of keloid.