Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (10): 1494-1500.doi: 10.3969/j.issn.2095-4344.2016.10.018

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Bone marrow mesenchymal stem cell transplantation improves airway inflammation due to chronic asthma

Zhang Qi, Guo Rui-rui, Hu Jiang-ping     

  1. Department of Respiratory Medicine, Zhengzhou Central Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Received:2016-01-18 Online:2016-03-04 Published:2016-03-04
  • About author:Zhang Qi, Attending physician, Department of Respiratory Medicine, Zhengzhou Central Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Supported by:

    the Medical Science Research Project of Hebei Province in 2014, No. ZD20140271

Abstract:

BACKGROUND: Studies have shown that bone marrow mesenchymal stem cell transplantation can improve disease conditions by reducing inflammation.
OBJECTIVE: To explore the therapeutic efficacy of bone marrow mesenchymal stem cells on chronic asthma rats.
METHODS: A rat model of chronic asthma was established by intraperitoneally injected and aerosolized ovalbumin. After modeling, rats were given 4×105 and 8×105 bone marrow mesenchymal stem cells via the tail vein, respectively. Thirty days later, the lung tissues were observed pathologically using hematoxylin-eosin staining; RT-qPCR and ELISA methods were employed to test the changes in interleukin-10, tumor necrosis factor-α and interferon-γ levels in lung tissue and peripheral blood, respectively.
RESULTS AND CONCLUSION: Rat models of chronic asthma were successfully established after intraperitoneal injection of ovalbumin combined with aerosolized ovalbumin. After 30 days of cell treatment, the structure of lung tissues were obviously recovered, and the levels of interleukin-10, tumor necrosis factor-α and interferon-γ showed some improvement in lung tissue and peripheral blood, but there were no differences between the two groups. In conclusion, bone marrow mesenchymal stem cells show some potential role in the treatment of chronic asthma.