Isoflurane inhibits neural stem cell proliferation in the hippocampus and promotes its differentiation into neurons

doi:10.3969/j.issn.2095-4344.2016.10.017

Abstract

Abstract:

BACKGROUND: Isoflurane cannot only induce a wide range of large neuronal apoptosis, but also inhibit hippocampal neurogenesis in neonatal rats, thereby resulting in hippocampus-dependent learning and memory defects.

OBJECTIVE: To investigate the isoflurane effect on proliferation and differentiation of the hippocampal neural stem cells.

METHODS: Twenty-six Sprague-Dawley rats were randomly divided into air group and isoflurane group (n=13 per group). Rats in the isoflurane group were subjected to 2.5% isoflurane inhalation for 3 minutes followed by 1.5% isoflurane inhalation for 4 hours. Rats in the air group only breathed in air. After the intervention, blood glucose and arterial blood gas changes were detected in the two groups. Additionally, rats in the two groups were given intraperitoneal injection of 5-bromodeoxyuridine before and after intervention. At 24 hours after the last injection of 5-bromodeoxyuridine, brain tissues were taken to make frozen sections for immunofluorescence staining.

RESULTS AND CONCLUSION: There were no significant difference in pH, PaO₂, PaCO₂, HCO₃, BE and SaO₂ levels between the two groups (P > 0.05). Compared with the air group, the number of BrdU⁺ cells was significantly less in the isoflurane group (P < 0.05), while the number of NeuroD⁺/BrdU⁺ cells was significantly higher in the isoflurane group (P < 0.05). The incidence of adverse reactions was 23% in the isoflurane group, which was significantly higher than that in the air group (7.7%; P < 0.05). These findings indicate that isoflurane can inhibit the proliferation of neural stem cells in the hippocampal dentate gyrus, and promote their differentiation into neurons.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Sun Nai, Li Chun-wei, Zhao Wei-xin, Song Qiong, Xia Gui-shan. Isoflurane inhibits neural stem cell proliferation in the hippocampus and promotes its differentiation into neurons[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(10): 1488-1493.

Figures/Tables 4

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