Stereotactic transplantation of neural stem cells into the brain improves motor function of craniocerebral trauma rats

doi:10.3969/j.issn.2095-4344.2016.10.011

Abstract

Abstract:

BACKGROUND: Cell replacement therapy as an effective strategy for reconstruction of the central nervous system has very broad application prospects.
OBJECTIVE: To investigate the effect of stereotactic transplantation of neural stem cells into the brain on the neuromotor function of craniocerebral trauma rats.
METHODS: Twenty male Sprague-Dawley rats were equivalently randomized into study and control groups. Animal models of craniocerebral trauma were made using the improved free-fall method in the rats. Then, model rats in the study and control groups were given parenchymal transplantation of embryonic neural stem cells and the same volume of culture medium with no stem cells at 1 day after injury, respectively. Neuromotor function of rats was assessed based on the neurological severity scores. At 2 weeks after transplantation, brain tissues were taken for hematoxylin-eosin staining, anti-BrdU, glial fibrillary acidic protein, β-tubulin III and tyrosine hydroxylase immunohistochemistry staining.
RESULTS AND CONCLUSION: The neurological severity scores in the study group were significantly lower than those in the control group at 1 and 2 weeks after injury (P < 0.05). In the study group, there were many BrdU-positive neural stem cells in the brain tissues, some of which were positive for glial fibrillary acidic protein, β-tubulin III and tyrosine hydroxylase; while in the control group, there was no BrdU-positive cell in the brain tissues. Experimental findings show that neural stem cells stereotactically transplanted into the brain can proliferate and differentiate in the brain lesion, and thereby notably improve the neuromotor function of rats with craniocerebral trauma.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Diao Xin-feng, Cheng Li-min, Xue Yong, Hu Cheng-wang, Cai Zhong-li. Stereotactic transplantation of neural stem cells into the brain improves motor function of craniocerebral trauma rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(10): 1446-1451.

Figures/Tables 3

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