Stromal cell derived factor-1 promotes the proliferation of bone marrow stem cells: the optimal concentration is 200 μg/L

doi:10.3969/j.issn.2095-4344.2016.10.005

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (10): 1402-1408.doi: 10.3969/j.issn.2095-4344.2016.10.005

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Stromal cell derived factor-1 promotes the proliferation of bone marrow stem cells: the optimal concentration is 200 μg/L

Peng Wei¹, Qin Yuan², Liao Chun-hui³, Chen Song-ling⁴

¹Department of Stomatology, ⁴Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China; ²Department of Emergency and Clinical Medicine, Hospital of Stomatology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China; ³Guangzhou Women and Children’s Medical Center, Guangzhou 510623, Guangdong Province, China

Received:2016-01-23 Online:2016-03-04 Published:2016-03-04
Contact: Chen Song-ling, M.D., Professor, Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China
About author:Peng Wei, Master, Physician, Department of Stomatology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China, No. 81371111; the Natural Science Foundation of Guangdong Province, No. 2014A030313059

Abstract

Abstract:

BACKGROUND: Stromal cell derived factor-1 is a small molecular protein with a wide range of biological activity that can cause immune cell chemotaxis, and it also has a chemotactic effect on bone marrow stem cells and periodontal ligament cells.
OBJECTIVE: To investigate the effect of stromal cell derived factor-1 with different concentrations on the proliferation of bone marrow stem cells and to probe the best concentration.
METHODS: Bone marrow stem cells from beagle dogs were cultured in vitro and stimulated by different concentrations of stromal cell derived factor-1 (100, 200, 300 μg/L). MTT was used to detect the influence of stromal cell derived factor-1 on the proliferation of bone marrow stem cells so as to screen the best concentration of stromal cell derived factor-1. Then, stromal cell derived factor-1 at the best concentrations was used to intervene the bone marrow stem cells, and MTT was used again to detect the proliferation of bone marrow stem cells.
RESULTS AND CONCLUSION: Stromal cell derived factor-1 at concentrations of 100, 200, 300 μg/L could promote the proliferation of bone marrow stem cells, and the effect was more notable at 200 and
300 μg/L but with no significant difference. Therefore, 200 μg/L was considered to be the best concentration of stromal cell derived factor-1 for intervention of bone marrow stem cells. Compared with the blank control group, 200 μg/L stromal cell derived factor-1 could significantly promote the proliferation of bone marrow stem cells. Taken together, stromal cell derived factor-1 can promote the proliferation of bone marrow stem cells, and its best concentration is 200 μg/L.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Peng Wei, Qin Yuan, Liao Chun-hui, Chen Song-ling. Stromal cell derived factor-1 promotes the proliferation of bone marrow stem cells: the optimal concentration is 200 μg/L[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(10): 1402-1408.

Figures/Tables 5

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Stromal cell derived factor-1 promotes the proliferation of bone marrow stem cells: the optimal concentration is 200 μg/L

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