Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (10): 1382-1388.doi: 10.3969/j.issn.2095-4344.2016.10.002

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Surface labeling of bone marrow mesenchymal stem cells by biotin-streptavidin

Yang Lin1, Luo Fu-li2, Li Yun1, Wen Jun1, Xu Yang3   

  1. 1Department of Nephrology, Jiangxi Provincial People’s Hospital, Jiangxi Provincial Key Laboratory of Kidney Diseases, Nanchang 330006, Jiangxi Province, China; 2Department of Nephrology, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, Jiangxi Province, China; 3Nanchang University School of Medicine, Nanchang 330000, Jiangxi Province, China
  • Received:2016-01-18 Online:2016-03-04 Published:2016-03-04
  • Contact: Li Yun, Chief physician, Department of Nephrology, Jiangxi Provincial People’s Hospital, Jiangxi Provincial Key Laboratory of Kidney Diseases, Nanchang 330006, Jiangxi Province, China
  • About author:Yang Lin, M.D., Chief physician, Department of Nephrology, Jiangxi Provincial People’s Hospital, Jiangxi Provincial Key Laboratory of Kidney Diseases, Nanchang 330006, Jiangxi Province, China Luo Fu-li, Master, Physician, Department of Nephrology, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, Jiangxi Province, China Yang Lin and Luo Fu-li contributed equally to this work.
  • Supported by:
    the National Natural Science Foundation of China, No. 81260115; the Natural Science Foundation of Jiangxi Province, No. 20122BAB205005

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Abstract:

BACKGROUND: Currently, there is a lack of efficient, non-invasive way to transplant stem cells to the target organ or tissue. Exploring a way to guide targeting transplantation of stem cells and to improve the efficiency of stem cell homing is now one of focuses in the field of stem cells research.
OBJECTIVE: To establish a simple and feasible method to chemically modify the cell surface using biotin-streptavidin reaction system, and to evaluate the efficiency of this method to label bone marrow mesenchymal stem cells (BMSCs) and its effects on cell biological functions.
METHODS: Passage 3 BMSCs were obtained by whole bone marrow culture method and verified by flow cytometry. Biotin, streptavidin, sulfonated biotin-N-hydroxy-succinimide were used to equip the adhesion molecule ligand, sialyated LewisX (SLeX), to the BMSCs surface. The labeling rate of BMSCs was assessed using fluorescence microscope, the vitality of BMSCs was evaluated by trypan blue staining, and the proliferation of BMSCs was evaluated by cell counting kit-8 assay. Adipogenic and osteogenic inductions were used to evaluate the effect of the method on the multi-differentiation function of BMSCs.
RESULTS AND CONCLUSION: After culture for 2 weeks, passage 3 BMSCs were obtained and confirmed by expressing CD90, CD29 and lack of CD34, CD45. Biotin, streptavidin, sulfonated biotin-N-hydroxy-succinimide were successfully used to equip sialyated LewisX (SLeX) to the BMSCs surface and had minor effects on the vitality, proliferation, and differentiation of BMSCs. This method was simple for surface modification and had a high modification rate of 88%. The homing of BMSCs modified by this method to the target organ or tissue could be greatly enhanced. Therefore, this method potentially could have extensive and important applications.