Adipose-derived mesenchymal stem cells regulate T cell immune status in allergic rhinitis

doi:10.3969/j.issn.2095-4344.2016.10.001

Abstract

Abstract:

BACKGROUND: Adipose-derived mesenchymal stem cells are a kind of pluripotent stem cells that have the potential of self-renewal and proliferation, and have low immunogenicity and immunomodulatory role.
OBJECTIVE: To study the effects of adipose-derived mesenchymal stem cells on T cell immune status of allergic rhinitis mouse models.
METHODS: Sixty mice were randomly assigned into six groups (sensitized/challenged/treatment): experimental group 1 was given ovalbumin/ovalbumin/high-dose adipose-derived mesenchymal stem cells, experimental group 2 given ovalbumin/ovalbumin/low-dose adipose-derived mesenchymal stem cells, experimental group 3 given ovalbumin/ovalbumin/PBS, experimental group 4 given ovalbumin/ovalbumin/0, and experimental group 5 given PBS/PBS/0, and normal control group given no treatment. In the former five groups, intraperitoneal injection of 200 μL ovalbumin sensitizing solution or PBS was conducted for basic sensitization at days 0, 7, 14; 20 μL ovalbumin challenging solution or PBS was given for challenging at days 15-19. In the former three groups, 0.1 mL of high-dose, low-dose adipose-derived mesenchymal stem cells or PBS was given via the tail vein, respectively, at days 20-22 after sensitization and challenge. At 48 hours after final treatment, ELISA was used to detect serum levels of interleukin-4, interleukin-6, interleukin-10 and interferon-γ, and fluorogenic quantitative PCR used to detect the mRNA expressions of these cytokines in the spleen. Migration of fluorescent-labeled adipose-derived mesenchymal stem cells in the nasal mucosa was observed under fluorescence microscope, and pathological changes of the nasal mucosa were observed through hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Compared with the experimental group 4, the levels of interleukin-4 and interleukin-6 in the serum and spleen were significantly lower in the experimental group 1 (P < 0.05), and the levels of interluekin-10 and interferon-γ levels were significantly increased (P <0.05); while in the experimental group, the levels of interleukin-6 were reduced significantly (P < 0.05), the levels of interleukin-10 was increased significantly (P < 0.05), but no changes were found in the levels of interleukin-4 and interferon-γ (P > 0.05). Fluorescent-labeled adipose-derived mesenchymal stem cells could migrate into the nasal mucosa, and the number of migrated cells was notably higher in the experimental group 1 than experimental group 2. Eosinophil infiltration in the nasal mucosa was remarkably alleviated in the experimental groups 1 and 2. These findings suggest that adipose-derived mesenchymal stem cells play a non-specific immunomodulatory effect dose-dependently by regulating Th1/Th2 immune imbalances and deficiencies of Treg cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Xiao Er-bin, Zhao Bao-jian, Zhang Chi . Adipose-derived mesenchymal stem cells regulate T cell immune status in allergic rhinitis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(10): 1373-1381.

Figures/Tables 3

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