Preparation and properties of a new human acellular dermal matrix

doi:10.3969/j.issn.2095-4344.2016.07.013

Abstract

Abstract:

BACKGROUND: Early vascularization is crucial for wound healing. A high-porosity, macrovoid allogeneic skin leads to the rapid vascularization and cellular infiltration.
OBJECTIVE: To obtain a new allogeneic skin product with high porosity, good cell permeability and good histocompatibility using an improved preparation method of human acellular dermal matrix.
METHODS: Cell components of healthy human skins were removed by the improved method and the traditional method, respectively. The improved method was to remove the subcutaneous fat, eliminate the epidermis (1 mol/L NaCl solution at 37 ℃ for 24 hours) followed by shaking processing (2% NaOH at 45 ℃ for 4 hours), and then, the solution was neutralized with PBS rinsing, dried and stored at 4 ℃ for standby. We detected the porosity and degradation time in vitro of the acellular dermal matrices prepared by two methods and the cytotoxicity of the material infiltration liquid on the adipose-derived stem cells. Hematoxylin-eosin staining was used for the detection of the cell residual, the integrity of collagen and cell biocompatibility. Scanning electron microscopy was used to detect the pore size.
RESULTS AND CONCLUSION: Both the two methods could completely remove the cell components, and maintain the integrity of the collagen scaffold. The porosity of acellular dermal matrix with the improved method was (93.22±0.99)%, which was significantly higher than that with the traditional method [(74.28±2.06)%; P < 0.001]. However, there was no significant difference in in vitro degradation time between the two kinds of acellular dermal matrices (P > 0.05). No obvious cytotoxicity of the acellular dermal matrix prepared with the improved method was detected. At 3-7 days of co-culture, the adipose-derived stem cells cultured on the acellular dermal matrix prepared with the improved method could penetrate the basement membrane to the deep dermis, while there was no obvious cell invasion and growth in the deep dermis prepared by the traditional method. Compared with the traditional method, the improved method is more suitable for cell infiltration and growth with higher porosity and larger pore size.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Jiang Tao, Zhang Ai-jun, Li Xue-yang, Ma Zhi-bing, Shen Cai-qi, Jin Pei-sheng . Preparation and properties of a new human acellular dermal matrix[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(7): 1006-1012.

Figures/Tables 5

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