Effect of simvastatin on bone mineral density and biomechanical properties of ovariectomized rats

doi:10.3969/j.issn.2095-4344.2016.07.009

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (7): 981-986.doi: 10.3969/j.issn.2095-4344.2016.07.009

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Effect of simvastatin on bone mineral density and biomechanical properties of ovariectomized rats

Zhang Yan¹, Liu Hao¹, Xing Lei¹, Zhang Guo-bin², Tian Fa-ming³

¹Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; ²Graduate School, ³Medical Research Center, North China University of Science and Technology, Tangshan 063000, Hebei Province, China

Received:2015-12-24 Online:2016-02-12 Published:2016-02-12
Contact: Tian Fa-ming, M.D., Associate professor, Master’s supervisor, Medical Research Center, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
About author:Zhang Yan, Master, Attending physician, Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
Supported by:
the Scientific Research Project of Hebei Universities and Colleges, No. QN20131007; the Natural Science Foundation of Hebei Province, No. H2013209255; Tangshan Key Laboratory Project of Geriatric Medicine, No. 14140221B

Abstract

Abstract:

BACKGROUND: Osteoporosis and its complications severely threaten the elder’s health. Simvastatin, widely accepted as a lipid-lowering drug, is reported to potentially promote bone formation, but it is in debate when orally administered, and there is no evidence to support whether this is due to the region difference.
OBJECTIVE: To investigate the effect of orally administered simvastatin on bone mass and biomechanical properties of the femur and vertebrae in osteopenia rats induced by ovariectomy (OVX).
METHODS: Twenty-four 6-month-old female Sprague-Dawley rats were subjected to OVX+orally administered saline vehicle (OVX group, n=8), OVX+orally administered simvastatin (5 mg/kg/d; intervention group, n=8) or sham surgery (sham group, n=8). After 8 weeks of treatment, all rats were sacrificed and the level of procollagen type I N-terminal propeptide in blood serum was assessed by ELISA. Bone mineral density was determined in the L5 vertebra and left femur using dual-energy X-rays. Furthermore, the biomechanical properties of the L4 vertebra and right femur, including maximum load and elastic modulus, were detected by compression testing and three-point bending test, respectively.
RESULTS AND CONCLUSION: The serum level of procollagen type I N-terminal propeptide in the sham group was significantly lower than that in the other two groups. OVX rats showed significantly lower bone mineral density in both the L5 vertebra and left femur than sham rats (P < 0.05). Rats in the intervention group showed higher bone mineral density than those in the OVX group, with statistically significant difference in the L5 vertebra (P < 0.05), but insignificant difference in the femur. Maximum load and elastic modulus of the L4 vertebra in the OVX group were significantly lower than those in the sham and intervention group. Markedly lower elastic modulus of the femur was found in the OVX group than the sham and intervention groups. These findings demonstrate that simvastatin treatment can partially prevent bone loss in OVX rats with more notable effect on the vertebrae than the femur, and for this model, the vertebra is superior to the femur used in biomechanical test.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Zhang Yan, Liu Hao, Xing Lei, Zhang Guo-bin, Tian Fa-ming. Effect of simvastatin on bone mineral density and biomechanical properties of ovariectomized rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(7): 981-986.

Figures/Tables 3

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Effect of simvastatin on bone mineral density and biomechanical properties of ovariectomized rats

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