Platelet-rich fibrin for repair of oral soft tissue defects

doi:10.3969/j.issn.2095-4344.2016.07.006

Abstract

Abstract:

BACKGROUND: Insufficient oral soft tissues in the implant zone may have a negative effect on the wound healing and the aesthetic restoration in the late stage. Platelet-rich fibrin can promote the wound healing of soft tissue defects. But there is still a lack of in-depth studies on the promotion of oral soft tissue defects in animal experiments.
OBJECTIVE: To compare the repairing effects of platelet-rich fibrin and collagen membrane on soft tissue defects of the hard palate in New Zealand rabbits.
METHODS: Fifty-four New Zealand rabbits were randomly divided into three groups (n=14 per group): platelet-rich fibrin group, collagen membrane group and blank control group. A 5 mm-diameter circular full-thickness soft tissue defect was made in the front of the hard palate, 2 mm distant to the rear maxillary incisors and mucosal edge of the bilateral hard palates. Autologous platelet-rich fibrin membrane or collagen membrane were implanted into the defect in the platelet-rich fibrin group and collagen membrane group, respectively. No treatment was given in the blank control group. General observation of the wound and wound healing analysis were performed at days 3, 7, 14, 21, 28, 56 post operation. Hematoxylin-eosin staining, CD31 immunohistochemical staining and Masson staining were used to observe inflammatory reaction, angiogenesis and collagen formation in the surgical site.
RESULTS AND CONCLUSION: The wound healing rate was fastest in the platelet-rich fibrin group, and no obvious scar formed. At 3 days post operation, there was no difference in the wound healing rates among the three groups; at 7 days, the wound healing rate in the platelet-rich fibrin group was significantly higher than that in the collagen membrane group and blank control group (P < 0.05). At 3 and 7 days after operation, the inflammatory reaction in the platelet-rich fibrin group was less than that in the collagen membrane and blank control groups (P < 0.05); at 14, 21, 28 and 56 days, there was no significant difference between the three groups. At 7, 14, 21 days after operation, the average absorbance value of CD31 in the platelet-rich fibrin group was significantly higher than that in the collagen membrane and blank control groups (P < 0.05). The average absorbance value of collagen formation in the platelet-rich fibrin group was significantly higher than that in the collagen membrane and blank control groups at 7 days after operation (P < 0.05), significantly higher than that in the blank control group at 14 days (P < 0.05), but lower than that in the collagen membrane and blank control groups at 21, 28 and 56 days after operation (P < 0.05). These findings show that platelet-rich fibrin can reduce inflammatory reactions in the process of wound healing, accelerate the angiogenesis, regulate the metabolism of collagen, reduce the formation of scar and improve the quality of wound healing, thereby promoting the repair of oral soft tissue defects.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Wang Tuo, Yang Qin-qiu, Dong Lu, Xiao Qiong, Chen Hong-liang, Sun Yong, Zhong Ke. Platelet-rich fibrin for repair of oral soft tissue defects[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(7): 957-965.

Figures/Tables 7

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