Endothelial progenitor cell transplantation combined with early exercise training for spinal cord injury: improvement in hindlimb function and angiogenesis in the injured region

doi:10.3969/j.issn.2095-4344.2016.06.018

Abstract

Abstract:

BACKGROUND: Endothelial progenitor cells are widely used in the treatment of various vascular diseases, and early exercise training contributes to restore motor function after spinal cord injury. However, the therapeutic effects of endothelial progenitor cell transplantation or early exercise training alone are unfavorable.
OBJECTIVE: To observe the influence of transplantation of endothelial progenitor cells combined with early exercise training on blood vessel regeneration and hind limb function in rats after spinal cord injury.
METHODS: Eighty adult Sprague-Dawley rats were enrolled to establish spinal cord injury models using the modified Allen’s method, and then randomly divided into four groups. Rats were respectively given culture medium via the tail vein, injection of endothelial progenitor cells (3×106) via the tail vein, roller and treadmill trainings for 2 weeks, or injection of endothelial progenitor cells via the tail vein followed by 2 weeks of roller and treadmill trainings in the model, cell transplantation, exercise and combined groups.
RESULTS AND CONCLUSION: At 2 weeks after transplantation, the hindlimb motor function of rats in the combined group was better than that in the cell transplantation group and exercise group, and moreover, the percentage of CM-Dil positive cells, the number of horseradish peroxidase-positive nerve fibers, capillary density and expression of vascular endothelial growth factor and brain-derived neurotrophic factor were also significantly higher in the combined group than the cell transplantation group and exercise group. These findings indicate that early exercise training has a neuroprotective role in spinal cord injury; endothelial progenitor cell transplantation combined with early exercise training can promote regeneration of synapses and blood vessels and improve hindlimb motor function of rats, probably by increasing expression levels of vascular endothelial growth factor and brain-derived neurotrophic factor.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Zhao Su-xiang, Hou Ying-nuo, Zhang Zi-tan, Liu Zhong-po, Nie Zhi-hong, Fan Ge-lin. Endothelial progenitor cell transplantation combined with early exercise training for spinal cord injury: improvement in hindlimb function and angiogenesis in the injured region[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(6): 883-890.

Figures/Tables 5

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