Effects of high-fat diet and treadmill exercise on glucose transporter 4 and cAMP response element binding protein in rat gastrocnemius muscle

doi:10.3969/j.issn.2095-4344.2016.05.022

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (5): 736-742.doi: 10.3969/j.issn.2095-4344.2016.05.022

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Effects of high-fat diet and treadmill exercise on glucose transporter 4 and cAMP response element binding protein in rat gastrocnemius muscle

Zhang Yun-li^{1, 2}, Cai Ming¹,Li Jing-jing¹, Xin Lei¹, Lou Shu-jie¹

¹School of Sports Science, Shanghai University of Sport, Shanghai 200438, China; ²School of Physical Education, Liaocheng University, Liaocheng 252059, Shandong Province, China

Received:2015-11-07 Online:2016-01-29 Published:2016-01-29
Contact: Lou Shu-jie, M.D., Professor, Doctoral supervisor, School of Sports Science, Shanghai University of Sport, Shanghai 200438, China
About author:Zhang Yun-li, Studying for doctorate, Lecturer, School of Sports Science, Shanghai University of Sport, Shanghai 200438, China; School of Physical Education, Liaocheng University, Liaocheng 252059, Shandong Province, China
Supported by:
the National Natural Science Founding of China, No. 81572241; the Shanghai Key Laboratory of Development and Protection of Human Exercise Capacity (Shanghai University of Sport), Shanghai, China, No. 11DZ2261100; the Graduate Education Innovation Grant Program in Shanghai University of Sport, Shanghai, China, No. xsxr2013030

Abstract

Abstract:

BACKGROUND: Some studies indicate that PI3K/Akt signaling pathway is associated with the expression of glucose transporter 4 (GLUT4) and the function of cAMP response element binding protein (CREB) in skeletal muscle. However, it is still unclear whether PI3K/Akt signaling pathway has the effects on CREB and GLUT4 in skeletal muscle of the rats with high-fat diet and treadmill exercise.
OBJECTIVE: To investigate whether PI3K/Akt signaling pathway has the effects on CREB and GLUT4 in gastrocnemius muscle of the rats with high-fat diet and treadmill exercise.
METHODS: A total of 70 rats were fed with normal diet for 2 weeks, and randomly divided into common feed group (n=20) and high-fat feed group (n=50). Rats in both groups were respectively fed with common feed and high-fat feed for 8 weeks. The rats in the common feed group were equally assigned to common feed quiet group and common feed exercise group. 20 rats from the high-fat feed group whose body weight was 1.4 times of common rats were randomly and equally assigned to obese quiet group and obese exercise group. Rats in the quiet groups did not do exercises. Rats in the exercise groups received adaptive sports for 1 week and medium-intensity treadmill exercise for 8 weeks.
RESULTS AND CONCLUSION: (1) Impairments of PI3K/Akt signaling pathway appeared in obese rats, however, the quantity of GLUT4 expression did not change obviously in gastrocnemius muscles of obese rats. The reasons for the decrease of the nuclear protein CREB level of gastrocnemius muscles of obese rats might be related to the decrease of pAkt-Ser473 level. (2) The increase of the quantity of GLUT4 expression was accompanied by significantly up-regulated pAkt-Ser473 level by exercise intervention in gastrocnemius muscles of obese rats. Exercise intervention significantly increased the expression of nuclear protein CREB in gastrocnemius muscles of chow-fed rats and obese rats, which was consistent with the changes of pAkt-Ser473. These findings suggest that pAkt-Ser473 can play an important role in the effects of high-fat diet and exercise intervention on GLUT4 and CREB protein expression in gastrocnemius muscles of obese rats.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Zhang Yun-li, Cai Ming,Li Jing-jing, Xin Lei, Lou Shu-jie. Effects of high-fat diet and treadmill exercise on glucose transporter 4 and cAMP response element binding protein in rat gastrocnemius muscle[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(5): 736-742.

Figures/Tables 5

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Effects of high-fat diet and treadmill exercise on glucose transporter 4 and cAMP response element binding protein in rat gastrocnemius muscle

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