Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (5): 707-711.doi: 10.3969/j.issn.2095-4344.2016.05.017

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Effect of coenzyme Q10 on the expression of interleukin-17 and interleukin-23 in gingival tissue of diabetic rats with periodontitis

Xu Yan-li, Xue Yi, Wu Zhong-yin, Zhang Nan   

  1. Bethune International Peace Hospital of PLA, Shijiazhuang 050082, Hebei Province, China
  • Received:2015-11-15 Online:2016-01-29 Published:2016-01-29
  • Contact: Xue Yi, Master, Chief physician, Bethune International Peace Hospital of PLA, Shijiazhuang 050082, Hebei Province, China
  • About author:Xu Yan-li, Master, Bethune International Peace Hospital of PLA, Shijiazhuang 050082, Hebei Province, China
  • Supported by:

    the General Program of “Twelfth Five-Year Plan” of Chinese PLA, No. CWS11J219

Abstract:

BACKGROUND: Coenzyme Q10 participates in the electron transport of respiratory chain and possesses antioxidant, anti-apoptotic and anti-inflammatory properties. It has achieved good outcomes in cardiovascular disease, diabetes and cancer. Coenzyme Q10 may also have a certain application value in the fields of diabetes and periodontitis.
OBJECTIVE: To observe the effect of coenzyme Q10 on the expression of interleukin-17 and interleukin-23 in gingival tissue of type 2 diabetic rats with periodontitis. 
METHODS: Forty-eight healthy male Wistar rats were randomly divided into 3 groups: control, periodontitis+ diabetes+physiological saline and periodontitis+diabetes+coenzyme Q10. Rats in the control group were fed with normal diet and water. Rats in the periodontitis+diabetes+physiological saline and periodontitis+diabetes+ coenzyme Q10 groups were subjected to induction of periodontitis using the method of silk ligation and type 2 diabetes by feeding a high-fat and high-sugar diet and intraperitoneal injection of streptozotocin. After successful modeling, rats in the periodontitis+diabetes+coenzyme Q10 group were intragastrically administered coenzyme Q10 for 8 successive weeks. Rats in the periodontitis+diabetes+physiological saline group were administered equal amount of physiological saline. At the end of 2nd, 4th and 8th weeks after drug administration, four rats were randomly selected and sacrificed. The expression levels of interleukin-17 and interleukin-23 in gingival tissue were detected by immunohistochemistry.
RESULTS AND CONCLUSION: At the end of 8th week, interleukin-17- and interleukin-23-positive expression in the periodontitis+diabetes+physiological saline group was significantly higher than that in the periodontitis+ diabetes+coenzyme Q10 group (P < 0.05). Coenzyme Q10 can reduce the expression levels of interleukin-17 and interleukin-23 in gingival tissue of type 2 diabetic rats with periodontitis, and alleviate periodontal tissue inflammation of type 2 diabetic rats with periodontitis.