Protective effect at low temperature of artificial cordyceps compound on isolated kidney from non-heart-beating rat models

doi:10.3969/j.issn.2095-4344.2016.05.010

Abstract

Abstract:

BACKGROUND: Non-heart beating donors have become the most promising source of donor in recent years. To further improve donor preservation solution, on the basis of conventional storage solution hypertonic citrate adenine solution, artificial cordyceps was added to prepare artificial cordyceps compound solution. It is hoped to reduce donor injury and to elevate donor quality by antioxidation.
OBJECTIVE: To investigate the preservation effect of artificial cordyceps compound solution on isolated renal from non-heart-beating rats.
METHODS: Medullas of 39 Sprague-Dawley rats were injured. Without in vitro respiratory and circulatory supports, non-heart-beating models were established and randomly divided into three groups: artificial cordyceps compound group (n=15), hypertonic citrate adenine solution group (n=15) and physiological saline group (n=9). The kidneys of rats in different groups were stored in artificial cordyceps compound solution, hypertonic citrate adenine solution and physiological saline at 4 ℃.
RESULTS AND CONCLUSION: Compared with the hypertonic citrate adenine solution group, after 6 and 12 hours of preservation, morphological injury was mild, apoptotic index, malondialdehyde, Bax and Caspase-3 expression levels decreased, superoxide dismutase activity and Bcl-2 expression increased; after 24 hours, the degree of injury was similar, malondialdehyde content decreased, and superoxide dismutase activity increased in the artificial cordyceps compound group. These findings indicated that the preservation effect of artificial cordyceps compound on antioxidation and inhibition of apoptosis was better than that of hypertonic citrate adenine solution in non-hearting beating rats.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Dai Chen, Wang Shun, Xu Jia-bo, Liu Jian. Protective effect at low temperature of artificial cordyceps compound on isolated kidney from non-heart-beating rat models[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(5): 664-670.

Figures/Tables 7

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