Effects of Tangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats

doi:10.3969/j.issn.2095-4344.2016.05.007

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (5): 646-651.doi: 10.3969/j.issn.2095-4344.2016.05.007

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Effects of Tangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats

Mu Xiao-hong¹, Sun Wen², Qin Ling-ling³, Wu Li-li⁴, Li Wei-li¹, Guo Xuan⁵, Zhang Lu⁵, Liu Tong-hua⁶

¹Department of Osteology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China; ²Health-Cultivation Laboratory of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, China; ³Department of Science and Technology, Beijing University of Chinese Medicine, Beijing 100029, China; ⁴Institute of Health Preserving of TCM, Beijing University of Chinese Medicine, Beijing 100029, China; ⁵Department of Endocrinology, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China; ⁶Graduate School of Beijing University of Chinese Medicine, Beijing 100029, China

Received:2015-11-07 Online:2016-01-29 Published:2016-01-29
Contact: Liu Tong-hua, M.D., Professor, Chief physician, Graduate School of Beijing University of Chinese Medicine, Beijing 100029, China
About author:Mu Xiao-hong, M.D., Professor, Chief physician, Department of Osteology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China
Supported by:
the National Natural Science Foundation of China, No. 81202970

Abstract

Abstract:

BACKGROUND: Chinese medicine Tangbikang can improve nerve conduction velocity in diabetic rats, and has anti-inflammatory and antioxidant effects. Insulin like growth factor-I is a key target in the treatment of diabetic peripheral neuropathy.
OBJECTIVE: To observe the effect of Tangbikang on the expression of insulin-like growth factor-I and its receptor protein and mRNA in the sciatic nerve of diabetic rat model.
METHODS: The experimental diabetes mellitus rat models were induced by feeding high fat forage and injection with streptozotocin. After model establishment, rats were given Tangbikang 4.18, 8.35, 16.7 mg/kg per day. This study set positive control methycobal, model and normal control groups. Intragastric administration was performed for 16 weeks.
RESULTS AND CONCLUSION: Compared with the model group, blood glucose levels were similar in the methycobal group, but decreased in the high-dose Tangbikang group (P < 0.01). Immunohistochemical staining and real-time fluorescence quantitative PCR detection revealed that body mass, motor nerve conduction velocity, insulin like growth factor-I and its receptor protein and mRNA expressions were increased in the methycobal and high-dose Tangbikang groups (P < 0.05 or P < 0.01). Results indicated that Tangbikang can prevent and treat diabetic peripheral neuropathy by promoting insulin like growth factor-I and its receptor.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Mu Xiao-hong, Sun Wen, Qin Ling-ling, Wu Li-li, Li Wei-li, Guo Xuan, Zhang Lu, Liu Tong-hua. Effects of Tangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(5): 646-651.

Figures/Tables 6

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Effects of Tangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats

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