Osteopontin promotes the expression of aggrecan and type II collagen in osteoarthritic chondrocytes of the knee joint in vitro

doi:10.3969/j.issn.2095-4344.2016.02.004

Chinese Journal of Tissue Engineering Research

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Osteopontin promotes the expression of aggrecan and type II collagen in osteoarthritic chondrocytes of the knee joint in vitro

Zhu He-yuan¹, Lei Guang-hua², Gao Shu-guang², Zhang Fang-jie², Zeng Chao², Zhang Kai²

¹Department of Orthopedics, Central Hospital of Loudi, Loudi 417000, Hunan Province, China; ²Department of Orthopedics, Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China

Received:2015-11-07 Online:2016-01-08 Published:2016-01-08
Contact: Lei Guang-hua, M.D., Professor, Department of Orthopedics, Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
About author:Zhu He-yuan, Master, Attending physician, Department of Orthopedics, Central Hospital of Loudi, Loudi 417000, Hunan Province, China
Supported by:
the National Natural Science Foundation of China, No. 8120420, 81272034, 81373130, 81501923; the Natural Science Foundation of Hunan Province, No. 14JJ3032

Abstract

Abstract:

BACKGROUND: Osteopontin mRNA and protein expressions are highly correlated with the severity of osteoarthritis.
OBJECTIVE: To investigate the effect of osteopontin on the gene expression of aggrecan and type II collagen in
the human knee osteoarthritic chondrocytes in vitro.
METHODS: Chondrocytes were harvested from human osteoarthritic knees and cultured in vitro. The chondrocytes were cultured with 0 (blank control group), 0.1, 1 mg/L osteopontin, respectively, for 48 hours. Real-time PCR was employed to detect the mRNA expression of aggrecan and type II collagen.
RESULTS AND CONCLUSION: After 0.1 and 1 mg/L osteopontin intervention, the mRNA expression of aggrecan and type II collagen in osteoarthritic chondrocytes was increased significantly (P < 0.05), and the mRNA expression of aggrecan and type II collagen was higher in the 1 mg/L osteopontin group than the 0.1 mg/L osteopontin group (P < 0.05). In addition, the mRNA expression of aggrecan and type II collagen was positively correlated with the concentration of osteopontin (r=0.751, P < 0.01; r=0.676, P < 0.01). These findings indicate that osteopontin up-regulates the mRNA expression of aggrecan and type II collagen in osteoarthritic chondrocytes of human knee in vitro in a dose-dependent manner.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Zhu He-yuan, Lei Guang-hua, Gao Shu-guang, Zhang Fang-jie, Zeng Chao, Zhang Kai. Osteopontin promotes the expression of aggrecan and type II collagen in osteoarthritic chondrocytes of the knee joint in vitro [J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2016.02.004.

Figures/Tables 5

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Osteopontin promotes the expression of aggrecan and type II collagen in osteoarthritic chondrocytes of the knee joint in vitro

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