Impact of perioperative cardiovascular risk factors on bone marrow progenitor cells

doi:10.3969/j.issn.2095-4344.2014.06.023

Abstract

Abstract:

BACKGROUND: Cell therapy by the implantation of autologous bone marrow cells has been used for the treatment of ischemic heart diseases in clinical trials for decade. However, as the outcomes of cell transplantation obviously vary among patients, it is essential to identify the risk factors that may influence the level and function of progenitor cells in bone marrow, in order to identify the patients who would benefit the most from this treatment.

OBJECTIVE: To observe the impact of perioperative cardiovascular risk factors on number and function of bone marrow progenitor cells from patients undergoing coronary artery bypass grafting surgery.

METHODS: We collected clinical and laboratory data from 44 patients scheduled to undergo sternotomy for coronary artery bypass grafting procedures. Bone marrow was aspirated from the sternum during the operation and bone marrow mononuclear cells were isolated by density centrifugation with Ficoll lymphoprep and then detected using trypan blue exclusion method. Levels of progenitor cells in bone marrow were evaluated using flow cytometry. Function of bone marrow progenitor cells were assessed by clonogenic and migration assays.

RESULTS AND CONCLUSION: We assessed the number of bone marrow mononuclear cells out of 20 mL bone marrow in duplicate samples from patients with coronary heart disease scheduled for coronary artery bypass grafting that was (10-89)×10⁶ cells with over 95% activity. A negative correlation was observed between the number of bone marrow mononuclear cells and the age (n=44, r=-0.788, P=0.001). Levels of CD34⁺, CD133⁺, and CD34⁺CD133⁺ cells in bone marrow mononuclear cells was (0.94±0.39)%, (0.46±0.28)%, and (0.53±0.26)%. Levels of CD34⁺ cells and CD133⁺ cells in patients with diabetes were significantly lower than those in patients without diabetes. Female, advanced age and poor heart function were related with reduced colony-forming ability of progenitor cells. A positive correlation was observed between level of CD34⁺ cells and migration ability of bone marrow mononuclear cells. The results show that by density gradient centrifugation, we can harvest a sufficient number of bone marrow mononuclear cells in the treatment for ischemic heart disease. Age, gender, diabetes, heart function are correlated with bone marrow mononuclear cell number and functions.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: coronary disease, heart diseases, myeloid progenitor cells, coronary artery bypass, off-pump

CLC Number:

R394.2

Zhang Lin, Gao Chang-qing, Wang Rong, Li Li-bing, Cheng Nan, Yao Ming-hui . Impact of perioperative cardiovascular risk factors on bone marrow progenitor cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(6): 962-967.

Figures/Tables 4

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