Nano-calcium titanate effects on rat osteoblast proliferation and differentiation

doi:10.3969/j.issn.2095-4344.2013.51.012

Abstract

Abstract:

BACKGROUND: Nano-calcium titanate, prepared with different modifiers, have properties of good osteogenesis, bone conductibility and biocompatibility.
OBJECTIVE: To observe the influence of nano-calcium titanate materials on the proliferation and differentiation of osteoblasts.
METHODS: Nano-calcium titanate was prepared with modifiers such as polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate. Meanwhile, nano-calcium titanate without modifier was prepared. All the four kinds of nano-calcium titanate materials were used to prepare nano-calcium titanate extracts under the concentration of 0.1, 1.0, 10 g/L, respectively. The proliferation of osteoblasts was observed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and alkaline phosphatase activity of osteoblasts was observed. Osteoblasts cultured in normal condition served as contrast.
RESULTS AND CONCLUSION: The proliferation of osteoblasts cultured in the calcium titanate extracts prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier was obviously improved under the concentration of 1.0 and 10 g/L. Absorbance value of the cells cultured in the above-mentioned four kinds of calcium titanate extracts 1.0 and 10 g/L) for 3, 6, 9 days exceeded the contrast (P < 0.05, P < 0.01), as well as the alkaline phosphatase activity (P < 0.05, P < 0.01). Calcium titanate prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier has no cytotoxicity effect on osteoblasts, and can promote the proliferation and differentiation of osteoblasts.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biomaterials, nanoparticles, osteoblasts, cell proliferation, mouth

CLC Number:

R318

Weng Zhi-qiang, Sun Min, Tang Xu-yan, Wu Zhan-ao, Duanli Rong, Jiang Tao. Nano-calcium titanate effects on rat osteoblast proliferation and differentiation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8856-8862.

Figures/Tables 7

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