Orthodontic force effects on the expression of periodontal interleukin-6 mRNA in rats

doi:10.3969/j.issn.2095-4344.2013.50.009

Abstract

Abstract:

BACKGROUND: As an important cytokine, interleukin-6 regulates immune responses in inflammation sites and has an autocrine/paracrine activity that stimulates osteoclast formation and bone resorption, which is related to bone remodeling during orthodontic tooth movement.
OBJECTIVE: To investigate the effects of orthodontic force on the expression of interleukin-6 mRNA in the periodontal tissue of rats.
METHODS: In situ hybridization was performed to measure the expression of interleukin-6 mRNA at 1, 3, 5, 7, 10 and 14 days after the application of orthodontic force on the maxillary first molars of rats.
RESULTS AND CONCLUSION: The expression of interleukin-6 mRNA was observed at a low level in the normal periodontal tissue of rats. After the application of force, the induction of interleukin-6 mRNA was observed to reach a maximum on day 3 and to decline thereafter. The expression of interleukin-6 mRNA can be evoked by orthodontic force but with a certain self-limiting. As a multifunctional cytokine, interleukin-6 plays a very important role in periodontal remodeling during orthodontic tooth movement.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: orthodontic extrusion, periodontium, interleukin-6, osteoclasts, alveolar bone loss

CLC Number:

R318

Qian Ya-jing, Nie Jing, Pan Xu, Hu Ming-hua, Mi Cong-bo. Orthodontic force effects on the expression of periodontal interleukin-6 mRNA in rats[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.50.009.

Figures/Tables 3

References

[1] Di Domenico M, D'apuzzo F, Feola A, et al. Cytokines and VEGF induction in orthodontic movement in animal models. J Biomed Biotechnol. 2012;2012:201689.

[2] Meikle MC. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. Eur J Orthod. 2006,28(3):221-240.

[3] Masella RS, Chung P. Thinking Beyond the Wire: Emerging Biologic Relationships in Orthodontics and Periodontology. Seminars in Orthodontics. 2008;14(4):290-304.

[4] Krishnan V, Davidovitch Z. Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop. 2006;129(4):461-469.

[5] Krishnan V, Davidovitch Z. On a path to unfolding the biological mechanisms of orthodontic tooth movement. J Dent Res. 2009;88(7):597-608.

[6] Meeran NA. Cellular response within the periodontal ligament on application of orthodontic forces. J Indian Soc Periodontol. 2013;17(1):16-20.

[7] Basaran G, Ozer T, Kaya FA, et al. Interleukins 2, 6, and 8 levels in human gingival sulcus during orthodontic treatment. Am J Orthod Dentofacial Orthop. 2006;130(1):1-7.

[8] Yang JH, Li ZC, Kong WD, et al. Effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats[J]. Asian Pac J Trop Med. 2013;6(10): 757-761.

[9] 潘旭,米丛波,刘红,等. 正畸牙移动过程中牙周组织白细胞介素8的表达[J]. 中国组织工程研究与临床康复,2011(20):3737- 3740.

[10] Alhashimi N, Frithiof L, Brudvik P, et al. Orthodontic tooth movement and de novo synthesis of proinflammatory cytokines. Am J Orthod Dentofacial Orthop.2001;119(3): 307-312.

[11] d'Apuzzo F, Cappabianca S, Ciavarella D, et al. Biomarkers of periodontal tissue remodeling during orthodontic tooth movement in mice and men: overview and clinical relevance. ScientificWorldJournal. 2013;2013:105873.

[12] 章捍东,刘洪臣. 白细胞介素-6与牙周组织的改建[J]. 牙体牙髓牙周病学杂志,2005,15(6):354-357.

[13] 蒲玉梅,高静,吴文蕾,等. IL-6在牙周炎中的作用及其调控因素[J]. 牙体牙髓牙周病学杂志, 2005,15(3):176-180.

[14] Okada H,Murakami S. Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med. 1998;9(3): 248-266.

[15] Balto K, Sasaki H, Stashenko P. Interleukin-6 deficiency increases inflammatory bone destruction. Infect Immun. 2001;69(2):744-750.

[16] Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis?. J Clin Periodontol. 2011;38 Suppl 11:60-84.

[17] 韩光丽,贺红,华先明,等. 大鼠牙移动性根吸收组织内组织蛋白酶K及白细胞介素6mRNA的表达[J].中华口腔医学杂志,2004, 39(4):59-62.

[18] 袁林,赵云凤,周伟东. IL-6表达蛋白与mRNA转录在咬合力影响大鼠牙周组织改建中的变化及意义[J].中华口腔医学杂志,2002, 37(1):54-57.

[19] 袁林,赵云凤,周伟东. 咬合力丧失影响大鼠牙周组织白细胞介素-6变化的研究[J]. 华西口腔医学杂志,2000,28(6):367-368.

[20] Uematsu S, Mogi M, Deguchi T. Interleukin (IL)-1 beta, IL-6, tumor necrosis factor-alpha, epidermal growth factor, and beta 2-microglobulin levels are elevated in gingival crevicular fluid during human orthodontic tooth movement. J Dent Res. 1996;75(1):562-567.

[21] Ren Y, Maltha J C, Van'T H M, et al. Cytokine levels in crevicular fluid are less responsive to orthodontic force in adults than in juveniles. J Clin Periodontol. 2002;29(8): 757-762.

[22] 姚艳丽,冯小平,经先振.正畸加力后牙周疼痛与龈沟液中生物活性物质变化的相关研究[J].上海口腔医学,2003,15(5): 331-333.

[23] Ren Y, Hazemeijer H, de Haan B, et al. Cytokine profiles in crevicular fluid during orthodontic tooth movement of short and long durations. J Periodontol.2007;78(3):453-458.

[24] Ren Y, Vissink A. Cytokines in crevicular fluid and orthodontic tooth movement. Eur J Oral Sci. 2008;116(2):89-97.

[25] Madureira DF, Taddei SA, Abreu MH, et al. Kinetics of interleukin-6 and chemokine ligands 2 and 3 expression of periodontal tissues during orthodontic tooth movement. Am J Orthod Dentofacial Orthop.2012;142(4):494-500.

[26] Grant M, Wilson J, Rock P, et al. Induction of cytokines, MMP9, TIMPs, RANKL and OPG during orthodontic tooth movement. Eur J Orthod. 2013;35(5):644-651.

[27] Maclaine JK, Rabie A, Wong R. Does orthodontic tooth movement cause an elevation in systemic inflammatory markers?. Eur J Orthod. 2010;32(4):435-440.

[28] Koyama Y, Mitsui N, Suzuki N, et al. Effect of compressive force on the expression of inflammatory cytokines and their receptors in osteoblastic Saos-2 cells. Arch Oral Biol. 2008; 53(5):488-496.

[29] 钱雅婧,钟良军,米丛波,等. 正畸力作用下大鼠炎性牙周组织改建及白细胞介素6的表达[J]. 中华口腔医学研究杂志(电子版), 2010,4(5):447-455.

[30] Chae HS, Park HJ, Hwang HR, et al. The effect of antioxidants on the production of pro-inflammatory cytokines and orthodontic tooth movement. Mol Cells. 2011;32(2): 189-196.