Effects of the L-type calcium channels on chondrocytes in response to basic fibroblast growth factor

doi:10.3969/j.issn.2095-4344.2013.50.006

Abstract

Abstract:

BACKGROUND: L-type calcium channels, as a kind of voltage-dependent calcium channel, is the main way of extracellular calcium ions into the cell, and play an important role in maintaining cell morphology and physiological activities, characterized by a large single-channel conductance, slow channel attenuation, and longer duration of channel opening. Previous studies showed that basic fibroblast growth factor can promote the proliferation of chondrocytes cultured in vitro.
OBJECTIVE: To explore the effect of the L-type calcium channels on regulating chondrocyte proliferation and differentiation in response to basic fibroblast growth factor with patch-clamp.
METHODS: The chondrocytes were harvested from the joints of 3-day-old New Zealand rabbits. The second passage of chondrocytes was divided into experimental group and control group. Chondrocytes were incubated in media containing 10 μg/L basic fibroblast growth factor and media alone separately. The opening of L-type calcium channels under the action of basic fibroblast growth factor was detected by patch-clamp. The intracellular calcium concentration was detected with laser confocal microscopy in the chondrocytes after 2 weeks of culture with basic fibroblast growth factor. Chondrocyte proliferation was analyzed by Cell Titer kit after 8 days of culture. Type Ⅱ collagen was assessed quantitatively by immunohistochemistrical staining after 10 days of culture.
RESULTS AND CONCLUSION: Basic fibroblast growth factor has an inhibitory effect on the opening of the L-type calcium channels, resulting in a decrease in intracellular free calcium concentration (P < 0.01). Cell number was higher after culture with basic fibroblast growth factor than that cultured under conventional condition (P < 0.01), and staining area of type II collagen significantly increased (P < 0.05). Results verified that basic fibroblast growth factor can maintain intracellular Ca2+ concentration at a low level by inhibiting the opening of L-type calcium channels, which can promote the proliferation and differentiation of chondrocytes.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: patch clamp, L-type calcium channel, chondrocytes, cell proliferation, cell growth factor

CLC Number:

R318

Wang Qiang, He Jin-shan, Xiong Chuan-zhi, Feng Xin-min, Wang Jing-cheng, Yan Lian-qi, Chen Peng-tao, Cai Jun. Effects of the L-type calcium channels on chondrocytes in response to basic fibroblast growth factor[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.50.006.

Figures/Tables 4

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