Mangiferin protects bone marrow-derived mesenchymal stem cells against hypoxia-induced apoptosis

doi:10.3969/j.issn.2095-4344.2013.49.004

Abstract

Abstract:

BACKGROUND: Hypoxic death limits application of cells in transplantation and tissue regeneration.
OBJECTIVE: To investigate the protective effects of mangiferin on bone marrow-derived mesenchymal stem cells against hypoxia injury-induced apoptosis resulted from cobalt chloride.
METHODS: Rat bone marrow-derived mesenchymal stem cells were in vitro cultured and hypoxia cell model was established by cobalt chloride. Model cells were treated with mangiferin. Protective effects of mangiferin were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; cell apoptosis and mitochondrial membrane potential were detected using flow cytometry.
RESULTS AND CONCLUSION: Cobalt chloride significantly inhibited growth of bone marrow-derived mesenchymal stem cells in a dose-dependent manner. The apoptosis rate of cells was (42.49±3.96)% after treated with 200 μmol/L cobalt chloride for 12 hours, (46.37±4.49)% after treated for 24 hours. With increasing concentration of mangiferin, apoptosis of bone marrow-derived mesenchymal stem cells in hypoxic model was gradually reduced (P < 0.01), indicating that mangiferin has a protective effect in a concentration-dependent manner on rat bone marrow-derived mesenchymal stem cells in hypoxic injury. Cobalt chloride can induce hypoxic model successfully in bone marrow-derived mesenchymal stem cells. There are some advantages of accurate dose control, no special equipment requirements, and easy operation. Mangiferin can effectively inhibit bone marrow-derived mesenchymal stem cells apoptosis under hypoxic injury.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, hypoxia, apoptosis, membrane potentials, mitochondria

CLC Number:

R394.2

Li Xiao-feng, Luo Shi-xing, Zhao Jin-min, Cheng Jian-wen, Tan Zhen. Mangiferin protects bone marrow-derived mesenchymal stem cells against hypoxia-induced apoptosis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8481-8487.

Figures/Tables 5

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