Morphology and distribution of CD44+/Oct4+ colorectal cancer stem cells

doi:10.3969/j.issn.2095-4344.2013.49.001

Abstract

Abstract:

BACKGROUND: More and more studies employ CD44 as a specific marker of colorectal cancer stem cells. Oct4 is a transcription factor of embryonic stem cells, and it has been discovered recently that there is a higher expression in primary colorectal carcinoma.
OBJECTIVE: To investigate the quantity, location and distribution of CD44⁺/Oct4⁺ cells in primary colorectal carcinoma.
METHODS: A totally of 108 cases of human colorectal carcinoma and 18 cases of normal mucosa, 18 cases of adenoma were collected and made into three tissue microarrays, each containing of 48 dots. The locations of CD44⁺/Oct4⁺ cells were detected by double-label immunohistochemical staining and hematoxylin-eosin staining. The morphologic features of them were investigated on hematoxylin-eosin staining at the same position.
RESULTS AND CONCLUSION: The results of double-label immunohistochemical staining demonstrated that there were no CD44⁺/Oct4⁺ cells in normal intestine mucosa and a very small amount of CD44⁺/Oct4⁺ cells in adenoma, and double-positive cells could also be seen in colorectal carcinoma. The number of CD44⁺/Oct4⁺ cells was rare and the cells were scattered or distributed focally along the basement of gland basal side. The cells with scarce cytoplasm were square, and its nucleus was oval or high cylindrical, deeply stained and homogeneous. The quantity of CD44⁺/Oct4⁺ cells was negatively correlated with the differentiation of colorectal cancer (r=-0.579, P < 0.01), and was associated with the depth of tumor invasion (r=0.236, P < 0.05). These findings indicate that CD44⁺/Oct4⁺ cells may be colorectal cancer stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, neoplastic stem cells, adenoma, antigens, CD44

CLC Number:

R394.2

Zhang Deng-cai, Liu Bin, Zhang Li-hua, Zhang Cai-lan, Yang Yan-li, Su Qin-jun, Shi Min, Dong Liang, Ha Ying-di . Morphology and distribution of CD44⁺/Oct4⁺ colorectal cancer stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8461-8467.

Figures/Tables 3

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