Preparation and cell compatibility of malleable demineralized bone matrix/hyaluronic acid putty

doi:10.3969/j.issn.2095-4344.2013.47.007

Abstract

Abstract:

BACKGROUND: It is important to prepare a malleable putty to repair irregular bone defects because the preformed bone substitute material cannot suit to bone defects.
OBJECTIVE: To prepare malleable demineralized bone matrix/hyaluronic acid putty and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility.
METHODS: Demineralized bone matrix was prepared from the cortical bone of healthy donors. The hyaluronic acid was dissolved at concentrations of 0.75%, 1.5%, 3%, 4.5%, and the viscosity of them was measured. Then 450 mg demineralized bone matrix was compounded with 1 mL hyaluronic acid solution to prepare the demineralized bone matrix/hyaluronic acid putty, and the optimal ratio of demineralized bone matrix/hyaluronic acid was screened by the disintegrated time of the putty at 37 ℃. L-929 mouse fibroblasts were cultured in the leaching liquor of demineralized bone matrix/hyaluronic acid, and the cytotoxicity was tested by cell count kit-8 assay at days 1, 4 and 7.
RESULTS AND CONCLUSION: With the increase of hyaluronic acid concentration, the viscosity of hyaluronic acid solution was increased; the demineralized bone matrix/hyaluronic acid putty was strengthened and the disintegrated time was prolonged gradually. When the ratio of demineralized bone matrix/hyaluronic acid was 15/1, demineralized bone matrix/hyaluronic acid was molded easily, and the disintegrated time was 8 hours in 7 ℃ PBS. The results of cytotoxicity showed that cell proliferation rates were 93.72%, 101.65% and 97.68% at days 1, 4 and 7 respectively, while the cytotoxicity was in grade 0 or 1. These findings indicate that demineralized bone matrix/hyaluronic acid putty at a compound ratio of 15:1 can be molded easily and difficult to disintegrate, with low cytotoxicity.

Key words: biocompatible materials, bone matrix, hyaluronic acid, histocompatibility testing

CLC Number:

R318

Zhang Nai-li, Zhang Yu-min, Zhou Mo, Yang Ting, Wang Xu-sheng, Ma Shao-ying, Xu Wei-jun, Dong Li,Li Bao-xing. Preparation and cell compatibility of malleable demineralized bone matrix/hyaluronic acid putty[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(47): 8182-8188.

Figures/Tables 5

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