Cytocompatibility and cytotoxicity of a novel composite bone cement material composed of chitosan microsphere, alpha-tricalcium phosphate and silk fibroin

doi:10.3969/j.issn.2095-4344.2014.16.010

Abstract

Abstract:

BACKGROUND: With excellent biocompatibility and osteoconduction, calcium phosphate bone cement has been used in clinic, but the poor mechanical properties and lack of osteoinduction restrict its further use.

OBJECTIVE: To investigate the cytocompatibility and cytotoxicity of a novel drug-carrying composite of bone cement composed of chitosan microsphere, α-tricalcium phosphate and silk fibroin.

METHODS: MC3T3-E1 cells were cultured in vitro in minimum essential medium alpha medium (α-MEM), which was supplemented with 10% fetal bovine serum, and 1% streptomycin sulfate, extract of the cement material at concentrations of 100% and 50%, and 6.4 mL/L phenol. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to measure cell proliferation and the cytotoxicity was assessed by the activity of lactate dehydrogenase. The MC3T3-E1 cells cultured in vitro were collected and seeded on the composite cement material, and cell morphology was observed by scanning electron microscope.

RESULTS AND CONCLUSION: The extract of composite cement material had no influences on the MC3T3-E1 cell proliferation, showing no obvious cytotoxicity. The scanning electron microscope image showed MC3T3-E1 cells adhered and proliferated well on the composite cement material composed of chitosan microsphere, α-tricalcium phosphate and silk fibroin, and pseudopodia out of the cells were closely attached to the material surface. In conclusion, the cement composite was proved to have satisfactory cytocompatibility and no obvious cytotoxicity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, calcium phosphates, chitosan, silk

CLC Number:

R318

Wang Jin-ning, Pi Bin, Wang Peng, Zhu Xue-song, Yang Hui-lin. Cytocompatibility and cytotoxicity of a novel composite bone cement material composed of chitosan microsphere, alpha-tricalcium phosphate and silk fibroin[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(16): 2519-2525.

Figures/Tables 2

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