Gene-transfected bone marrow mesenchymal stem cells combined with allogeneic bone for repair of sheep critical-size bone defects

doi:10.3969/j.issn.2095-4344.2013.47.001

Abstract

Abstract:

BACKGROUND: Many in vivo and in vitro experiments indicate that implantation of exogenous basic fibroblast growth factor can significantly promote the process of bone formation, but the in vivo degradation of exogenous basic fibroblast growth factor affects the therapeutic efficacy.
OBJECTIVE: To observe the effects of basic fibroblast growth factor-transfected mesenchymal stem cells which transfected using molecular biology techniques combined with allogeneic bone in the repair of critical-size bone defects in sheep.
METHODS: Allogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells, bone marrow mesenchymal stem cells combined with allogeneic bone material stents, allograft bone material, β-tricalcium calcium material were respectively implanted into critical-size bone defects in sheep. After 4, 8 and 12 weeks, histological and immunohistochemical staining was performed.
RESULTS AND CONCLUSION: At 12 weeks after implantation of allogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells as tissue engineering bone, there were many cartilage-like structures in the operative binding region and a large amount of osteoblast-like cells in the center of operative region, and there was more material degradation in the entire operative area as compared with other groups; there were fibrous connective tissues full of the pores, and osteoclast-like cells were commonly seen around the implant material; bone sialoprotein and collagen type Ⅰ expression were strongly positive. In the other three groups, although the cartilage-like structure appeared in the binding region, dead bone structure was found in the central area, and bone sialoprotein and type Ⅰ collagen expression was weak. These findings indicate that allogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells can basically repair critical-size bone defects in sheep.

Key words: biocompatible materials, bone marrow, stem cells, fibroblast growth factor 2, transfection

CLC Number:

R318

Wang Xiao-zhi, He Hui-yu, Yang Nan, Yang Ze-hui, Hu Yang. Gene-transfected bone marrow mesenchymal stem cells combined with allogeneic bone for repair of sheep critical-size bone defects[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(47): 8141-8148.

Figures/Tables 5

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