Abstract:

BACKGROUND: The studies have found that curcumin preconditioning and ischemic postconditioning have protective effect on renal ischemia-reperfusion injury.
OBJECTIVE: To establish a model of renal ischemia-reperfusion injury and observe the protective effect of curcumin preconditioning, ischemic postconditioning and curcumin preconditioning combined with ischemic postconditioning on the renal ischemia-reperfusion injury of rats.
METHODS: Sixty Sprague Dawley rats were randomly divided into five groups, namely sham-operation group, renal ischemia-reperfusion model group, curcumin preconditioning group, ischemic postconditioning group and curcumin preconditioning combined with ischemic postconditioning group (combination group). After reperfusion for 24 hours, the inferior vena venous blood was extracted to determine the values of blood creatinine and urea nitrogen. The superoxide dismutase activity and malondialdehyde value were measured in renal tissue, and hematoxylin-eosin staining was used to observe the changes in renal structure.
RESULTS AND CONCLUSION: Compared with sham-operation group, the values of blood creatinine and urea nitrogen in other four groups were significantly increased (P < 0.05). Compared with ischemia-reperfusion model group, the values of blood creatinine and urea nitrogen in the urcumin preconditioning group, ischemic postconditioning group and combination group were significantly decreased after reperfusion for 24 hours (P < 0.05). Compared with urcumin preconditioning group and ischemic postconditioning group, the blood creatinine value of the combination group was significantly lower (P < 0.05). Compared with the sham-operation group, superoxide dismutase activity in the ischemia-reperfusion model group was decreased, while the value of malondialdehyde was increased significantly, the differences were statistically significant (P < 0.05). Compared with ischemia-reperfusion model group, urcumin preconditioning group, ischemic postconditioning group and combination group had higher superoxide dismutase activity, while the value of malondialdehyde was decreased; the differences were statistically significant (P < 0.05). Compared with urcumin preconditioning group and ischemic postconditioning group, superoxide dismutase activity of the combination group was significantly increased, and the difference was significant (P < 0.05). Compared with the sham-operation group, the renal tubular injury of ischemia-reperfusion injury model group was obvious; compared with ischemia-reperfusion model group, the urcumin preconditioning group, ischemic postconditioning group and the combination group had the significantly reduced tubular injury, and compared with urcumin preconditioning group and ischemic postconditioning group, the combination group had more significantly reduced tubular injury. Our study suggests that curcumin preconditioning combined with ischemic postconditioning has a synergistic effect; it can better protect the kidney from ischemia-reperfusion injur