Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7539-7543.doi: 10.3969/j.issn.2095-4344.2012.40.024

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Differential proteomic research of liver ischemia-reperfusion injury undergoing resection of hepatocellular carcinomas

Huang Chuan-zhong1, Lin Ke-can2, Wang Bin3, Huang Ai-min3, Chen Hui-jing1, Tao Xuan3, Liu Jing-feng2, Ye Yun-bin1   

  1. 1Fujian Provincial Key Laboratory of Tumor Translational Medicine, Laboratory of Tumor Immunology, Fujian Provincial Tumor Hospital, Fuzhou 350014, Fujian Province, China
    2Department of Liver Surgery, Liver Center of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China
    3Department of Pathology, School of Basic Medical Science, Fujian Medical University, Fuzhou 350004, Fujian Province, China
  • Received:2012-01-28 Revised:2012-03-19 Online:2012-09-30 Published:2012-09-30
  • Contact: Ye Yun-bin, Doctor, Professor, Chief physician, Fujian Provincial Key Laboratory of Tumor Translational Medicine, Laboratory of Tumor Immunology, Fujian Provincial Tumor Hospital, Fuzhou 350014, Fujian Province, China yunbin_ye@yahoo.com Liu Jing-feng, Professor, Doctoral supervisor, Chief physician, Department of Liver Surgery, Liver Center of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China drjingfeng@yahoo.com.cn
  • About author:Huang Chuan-zhong★, Master, Fujian Provincial Key Laboratory of Tumor Translational Medicine, Laboratory of Tumor Immunology, Fujian Provincial Tumor Hospital, Fuzhou 350014, Fujian Province, China Hcz_2001@sina.com

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Abstract:

BACKGROUND: The ischemia reperfusion injury undergoes resection of hepatocellular carcinomas may promote the occult metastatic invasion and metastasis, and induce mitochondrial damage.
OBJECTIVE: To reveal the protein expression changes of normal liver tissue after ischemia reperfusion injury undergoes resection of hepatocellular carcinomas, and to screen the hepatic ischemia reperfusion injury stress related protein and to explore the possible mechanism.
METHODS: Normal liver tissue proteins and mitochondrial protein expression profiles were established in 20 liver cancer patients with hepatic ischemia reperfusion before and after perfusion for 15 minutes by isoelectric focusing/polyacrylamide gel electrophoresis, and the difference was analyzed. The matrix-assisted laser desorption inoization-time of flight mass spectrometry was used to identified different expression proteins, and the bioinformatic analysis was performed.
RESULTS AND CONCLUSION: Under the same condition, two-dimensional electrophoresis of each whole cell and mitochondrial protein samples were performed for three times. Different proteins appeared in each pair profile were analyzed. In the whole cell protein profiles, a total of 5 different proteins were identified and no significant difference of protein in mitochondrial profiles was identified. Liver ischemia reperfusion 15 minutes during hepatocellular carcinoma resection operation can change the expression of the cell protective protein such as cytoskeletal proteins and heat shock proteins, and can promote tumor metastasis risk and the preventive measures should be taken to reduce the impact. However, no changes happen in mitochondrial.

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