Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7533-7538.doi: 10.3969/j.issn.2095-4344.2012.40.023

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Effects of tetramethylpyrazine and silencing transforming growth factor-beta 1 by small interfering RNA on expression of connective tissue growth factor of rat hepatic stellate cells

Xiang Ying1, Li Jun2, Jia Qian1, Yang Hong1, Li Xiao-sheng1   

  1. 1Department of Gastroenterology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
    2Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China
  • Received:2012-02-25 Revised:2012-04-19 Online:2012-09-30 Published:2012-09-30
  • Contact: Li Xiao-sheng, Master, Professor, Master’s supervisor, Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China LXS6896@126.com
  • About author:Xiang Ying★, Studying for master’s degree, Department of Gastroenterology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China xiangyinga@126.com

Abstract:

BACKGROUND: Our previous studies have demonstrated that tetramethylpyrazine can anti-hepatic fibrosis via inhibiting hepatic stellate cells proliferation, inhibiting p38 mitogen-activated protein kinase signal pathway, as well as down-regulating the expression of connective tissue growth factor, and so on. Generally, transforming growth factor-β1 is the foremost cell factor in promoting hepatic fibrosis, and connective tissue growth factor is the downstream effect medium of transforming growth factor-β1, small interfering RNA targeting transforming growth factor-β1 can be a new way in the treatment of hepatic fibrosis.
OBJECTIVE: To investigate the effect of tetramethylpyrazine and chemosynthesis small interfering RNA transforming growth factor-β1 on the expression of connective tissue growth factor of rat hepatic stellate cells.
METHODS: Hepatic stellate cells were cultured in vitro, an effective segment of gene silencing was screened out from three segments of small interfering RNA transforming growth factor-β1, then it was transfection group utilizing lipsome transfection reagent to transfect together the cells which have been cultured for 24 hours, and blank control group, transfection reagent group, tetramethylpyrazine group, and union treatment group were designed.
RESULTS AND CONCLUSION: Compared with blank control group, the expression of collagen type Ⅰ, collagen type Ⅲ and connective tissue growth factor mRNA were reduced in transfection group, tetramethylpyrazine group and union treatment group (P < 0.05). The connective tissue growth factor protein expression was down-regulated in transfection group, tetramethylpyrazine group and union treatment group (P < 0.05); contents of collagen type Ⅰ and collagen type Ⅲ in the supernatant were reduced (P < 0.05). Result show that the small interfering RNA silencing transforming growth factor-β1 can reduce the expression of collagen type Ⅰ and collagen type Ⅲ and down-regulate the expression of connective tissue growth factor. The expression of connective tissue growth factor can be down-regulated by tetramethylpyrazine, but it is more pronounced to the inhibiting expression of collagen type Ⅰ and collagen type Ⅲ, cluing that the anti-hepatic fibrosis of tetramethylpyrazine may be multifarious action targets.

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