Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7528-7532.doi: 10.3969/j.issn.2095-4344.2012.40.022

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Expression of tight junction protein Occludin in chorionic villus and its significance

Feng Ya-ling1, 2, 3, Qi Guan-nan3, Zhou Chang-ju1   

  1. 1Department of Obstetrics and Gynecology, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
    2Department of Obstetrics and Gynecology, Shenzhen Baoan District People’s Hospital, Shenzhen 518101, Guangdong Province, China
    3Third Affiliated People's Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
  • Received:2012-01-23 Revised:2012-02-14 Online:2012-09-30 Published:2012-09-30
  • Contact: Zhou Chang-ju, Chief physician, Department of Obstetrics and Gynecology, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China zhouchanju8816@yahoo.com.cn
  • About author:Feng Ya-ling☆, Doctor, Associate chief physician, Department of Obstetrics and Gynecology, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China; Department of Obstetrics and Gynecology, Shenzhen Baoan District People’s Hospital, Shenzhen 518101, Guangdong Province, China; Third Affiliated People's Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China fengyl523@yahoo.com.cn

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Abstract:

BACKGROUND: The role of Occludin protein in the embryo implantation during early pregnancy has never been reported.
OBJECTIVE: To investigate the expression of Occludin in the chorionic villus and to explore its significance.
METHODS: The mRNA and protein expression of Occludin in chorionic villus from 30 normal women of early pregnancy and 30 missed abortion patients were measured by semi-quantitative reverse transcription-PCR, Western blot and immunohistochemistry methods.
RESULTS AND CONCLUSION: Occludin was showed in endochylema, cell nucleus and little stroma of syneytiotrophoblast cell of external chorionic villus and showed low expression in the cytotrophoblast. Semi-quantitative reverse transcription-PCR, Western blot and immunohistochemistry staining found that the Occludin expressed in chorionic villus in normal early pregnancy women and missed abortion patients, the expression in normal early pregnancy was lower than that in missed abortion patients, but there was no significant difference (P > 0.05). There was expression of Occludin in the chorionic villus. The lower expression of Occludin in cytotrophoblast might be one of the reasons for the polarity disappearance of cytotrophoblast cell and participated in the formation of infiltrated capacity of cytotrophoblast. Inadequate cytotrophoblast invasion ability was not the only reason for the embryo development stagnation.

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