Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7514-7519.doi: 10.3969/j.issn.2095-4344.2012.40.020

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Effects of erythropoietin on renal tubulointerstitial fibrosis after renal ischemia-reperfusion injury in mice

Chen Ying1, Wu Duo-jiang2, Yang Yi-bin1, Ke Gui-bao1, Zhang Fu-xiang1, Rong Song3   

  1. 1Department of Nephrology, the Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
    2Department of Hemodialysis, Pixian People’s Hospital, Chengdu 611730, Sichuan Province, China
    3Organ Transplantation Center, Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Received:2012-05-14 Revised:2012-07-20 Online:2012-09-30 Published:2012-09-30
  • Contact: Rong Song, Doctor, Professor, Doctoral supervisor, Organ Transplantation Center, Zunyi Medical College, Zunyi 563003, Guizhou Province, China songrong@hotmail.com
  • About author:Chen Ying★, Studying for master’s degree, Department of Nephrology, the Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China yingzi86817@163.com

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Abstract:

BACKGROUND: Erythropoietin can relieve inflammation, anti-apoptosis and have a protective effect on renal ischemia-reperfusion induced injury.
OBJECTIYE: To investigate effect of erythropoietin on apoptosis and renal tubulointerstitial fibrosis renal ischemia-reperfusion-induced injury in mice.
METHODS: The tubulointerstitial fibrosis model was established by unilateral renal ischemia-reperfusion injury. All mice were divided into four groups: sham-operation group, ischemia-reperfusion group, low and high doses erythropoietin group. The renal pathological changes were observed by hematoxylin-eosin staining and Masson staining. Meanwhile, the expression of Bcl-2 and Bax protein in renal tissue was assessed by immunohistochemical method. The expression of Capase-3 was analyzed by Western Blot.
RESULTS AND CONCLUSION: Compared with ischemia-reperfusion group, the pathological changes of tubules and interstitium in low and high doses erythropoietin groups were significantly improved. Compared with the sham-operation group, the levels of Bcl-2 and Bax expression were remarkably increased in ischemia-reperfusion group and erythropoietin groups, and most significant in ischemia-reperfusion group; the ratio of Bcl-2/Bax in ischemia-reperfusion group was remarkably lower than that in the sham-operation group, and the ratio in the erythropoietin groups was higher than that in the sham-operation group. The Caspase-3 expression in erythropoietin groups was significantly higher than that in the ischemia-reperfusion group, but lower than that in the sham-operation group. It shows that the development of renal tubulointerstitial fibrosis after ischemia-reperfusion induced injury is related with the cell apoptosis, and the expression of Bcl-2/Bax and Caspase-3 plays an important role. Low dose erythropoietin can attenuate the renal tubulointerstitial fibrosis after renal ischemia-reperfusion injury.

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