Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7508-7513.doi: 10.3969/j.issn.2095-4344.2012.40.019

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Effects of tetramethylpyrazine in rats with unilateral ureteral obstruction renal fibrosis

Li Jian-zhi1, Yu Jiang-dong2, Liang Yu3, Liu Yu-ming2, Hu Li1, Yang Bo4, Zhou Xiu-tian3   

  1. 1The Nursery College of Nanhua University, Hengyang 421001, Hunan Province, China
    2Hengyang Central Hospital, Hengyang 421008, Hunan Province, China
    3Medical College of Nanhua University, Hengyang 421001, Hunan Province, China
    4The First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China
  • Received:2012-03-01 Revised:2012-03-23 Online:2012-09-30 Published:2012-09-30
  • About author:Li Jian-zhi★, Master, Associate professor, the Nursery College of Nanhua University, Hengyang 421001, Hunan Province, China

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Abstract:

BACKGROUND: Renal interstitial fibrosis is related to the imbalance between matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2.
OBJECTIVE: To establish the unilateral ureteral obstruction renal fibrosis model and to observe the pathological changes of renal interstitial and the expression of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 after treated with tetramethylpyrazine.
METHODS: Twenty-four female Sprague Dawley rats were randomly divided into three groups: sham-operation group, model group and tetramethylpyrazine group. The rats in the model group and tetramethylpyrazine group were performed with left ureter ligation to establish the unilateral ureteral obstruction model. Rats in the tetramethylpyrazine group were performed with intragastric administration at 1 day before surgery, 40 mg/(kg•d) once per day and lasted for 2 weeks. All rats were sacrificed at 14 days after surgery. The pathological changes of the obstruction renal tissues were examined by hematoxylin-eosin staining and Masson staining. Immunohistochemistry and reverse transcription-polymerase chain reaction were applied to detect the protein and mRNA expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2.
RESULTS AND CONCLUSION: Tetramethylpyrazine could significantly reduce the expansion and contraction of the tubular, the proliferation of renal interstitial fibrous tissue and inflammatory cell infiltration. Compared with sham-operation group, the protein and mRNA expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2 was significantly increased in model group (P < 0.01). The protein and mRNA expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2 in tetramethylpyrazine group was smaller than that in the model group (P < 0.01). Tetramethylpyrazineon could relieve the renal intersitial fibrosis by decreasing expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2.

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