Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7503-7507.doi: 10.3969/j.issn.2095-4344.2012.40.018

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Rhein effects on apoptosis of glomerular mesangial cells cultured with high glucose in Sprague Dawley rats

Guo Yu, Li Yan, Fang Hui, Yin Xiao-xing, Wei Qun-li   

  1. College of Pharmacy, Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China
  • Received:2012-02-06 Revised:2012-02-14 Online:2012-09-30 Published:2012-09-30
  • Contact: Wei Qun-li, Doctor, Professor, Chief physician, College of Pharmacy, Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China 15077860033@139.com
  • About author:Guo Yu★, Studying for master’s degree, College of Pharmacy, Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China guoyu19861230@yahoo.com.cn

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Abstract:

BACKGROUND: Our previous study has already manifested that Chinese medicine Xiaokening containing Rhein can effectively control the development of early diabetic nephropathy.
OBJECTIVE: To investigate the effect of Rhein on apoptosis of glomerular mesangial cells (GMCs) of Sprague Dawley rats cultured with high glucose.
METHODS: The GMCs cultured with high glucose were stimulated by different concentrations of Rhein (20, 40, 80 μmol/L). Morphology of GMCs was discriminated by hematoxylin-eosin staining. Karyon apoptosis was examined by fluorescence staining of 4’,6-diamidino-2-phenylindole (DAPI). Cell apoptosis rate was detected by flow cytometry.
RESULTS AND CONCLUSION: The outcome of hematoxylin-eosin staining and 4’,6-diamidino-2-phenylindole staining indicated that Rhein could obviously induce apoptosis of GMCs in a dose- and time-dependent manner. The apoptosis rate had significant difference among different groups (P < 0.05). This result shows that Rhein can obviously induce apoptosis of GMCs cultured with high glucose and in a dose- and time-dependent manner.

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