Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7496-7502.doi: 10.3969/j.issn.2095-4344.2012.40.017

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Icariin alleviates renal ischemia-reperfusion injury in rats through inhibition of inflammation

Feng Gui-wen1, Liu Long-shan2, Shang Wen-jun1   

  1. 1The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
    2Department of Organ Transplantation, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Received:2012-06-08 Revised:2012-06-18 Online:2012-09-30 Published:2012-09-30
  • Contact: Shang Wen-jun, Doctor, Associate chief physician, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China shangwj111@163.com
  • About author:Feng Gui-wen☆, Doctor, Associate chief physician, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China fengguiwen@hotmail.com

Abstract:

BACKGROUND: The renal ischemia-reperfusion injury is inevitable during renal transplantation, reducing the damage by drugs can help to improve the short- and long-term efficacy of renal transplantation. Icariin has a good effect on the treatment of myocardial and cerebral ischemia, but the effect on the renal ischemia reperfusion is not clear.
OBJECTIVE: To investigate the protection mechanism of icariin on renal ischemia-reperfusion injury.
METHODS: Fifty-four Sprague Dawley rats were randomly divided into sham-operation group, icariin group and model group, rats in the icariin group and model group were used to establish the unilateral renal ischemia reperfusion injury model, and rats in the sham-operation only received the renal pedicle freeing without blocking the renal vascular. In icariin group and model group, icariin (100 mg/kg) and 0.3% sodium carboxymethyl cellulose (1 mL/kg) were orally administrated by gavage daily from 2 days before modeling to 12 days after modeling.
RESULTS AND CONCLUSION: Compared with sham-operation group, the serum creatinine level was significantly increased and the creatinine clearance rate was decreased after renal ischemia-reperfusion injury in icariin group and model group, and the decreased creatinine clearance rate resulted in the pathological injury of renal tissue. In the icariin group, the expression level of inducible nitric oxide synthase mRNA and protein was decreased, and the expression levels of pro-inflammatory cytokines (tumor necrosis factor α, interleukin-1β, interleukin 6), chemokines (interferon-inducible protein 10, monocyte chemoattractant protein 1, macrophage cell inflammatory protein-2) mRNA in the renal tissue were decreased; at 6 hours after renal ischemia-reperfusion injury, the content of plasma nitrite/nitrate and myeloperoxidase in icariin group reached to peak and then decreased gradually; the content of plasma nitrite/nitrate and myeloperoxidase in icariin group was lower than that in the model group (P < 0.05). Icariin can attenuate renal ischemia-reperfusion injury through inhibiting inducible nitric oxide synthase expression and downstream inflammation cascades.

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