Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7486-7490.doi: 10.3969/j.issn.2095-4344.2012.40.015

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Establishing a C57BL/6J mouse immunosuppressive model induced by cyclophosphamide

Yang Xian-yong   

  1. Taian City Central Hospital, Taian 271000, Shandong Province, China
  • Received:2012-06-08 Revised:2012-06-18 Online:2012-09-30 Published:2012-09-30
  • About author:Yang Xian-yong★, Master, Technician- in-charge, Taian City Central Hospital, Taian 271000, Shandong Province, China yang_xianyong@yahoo.com.cn

Abstract:

BACKGROUND: The immunosuppressive models are commonly used in the research of immunostimulants, but how to establish the immunosuppressive models is the key for the evaluation of immunostimulants.
OBJECTIVE: To establish C57BL/6J mice immunosuppressive model induced by cyclophosphamide.
METHODS: Normal C57BL/6J mice were randomly divided into six groups (n=4). Group 1 was normal control group, group 2 was injected with 50 mg/kg cyclophosphamide for 5 days, group 3 was injected with 80 mg/kg cyclophosphamide for 3 days, group 4 was injected with 80 mg/kg cyclophosphamide for 5 days, group 5 was injected with 100 mg/kg cyclophosphamide for 5 days, and group 6 was injected with 100 mg/kg cyclophosphamide every 2 days. Mice in group 1 were intraperitoneal injected with 0.1 mL normal saline and lasted for 5 days. Mice in group 2, 3, 4, 5 were intraperitoneal injected with 50, 80, 80 and 100 mg/kg cyclophosphamide, respectively, and lasted for 5, 3, 5 and 5 days, respectively. Mice in group 6 were intraperitoneal injected with 100 mg/kg cyclophosphamide every two days and total injected for three times.
RESULTS AND CONCLUSION: Compared with group 1, the CD3+T, CD3+CD4+T and CD3+CD8+T levels in the peripheral blood in the other five groups were significantly decreased (P < 0. 05); the contents of alanine aminotransferase (except for group 2 and group 3), aspartate aminotransferase and blood urea nitrogen in the other five groups were significantly increased (P < 0. 05), especially in group 4, 5 and 6. Intraperitoneal injection of cyclophosphamide can establish the immunosuppressive model with decreased level of CD3+T, CD3+CD4+T and CD3+CD8+T, and intraperitoneal injection of 50 mg/kg and 80 mg/kg cyclophosphamide for 5 and 3 days has less kidney and liver damage.

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