Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7475-7479.doi: 10.3969/j.issn.2095-4344.2012.40.013

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Effects of lycium barbarum polysaccharides on fetal ovarian tissue cryopreservation

Cai Yu-fang1, 2, Wang Yan-rong1, 2, Kong Bing1, Cui Xiu1, Shen Xin-sheng1   

  1. 1Department of Human Anatomy and Histology-Embryology, Ningxia Medical University Yinchuan 750004, Ningxia Hui Autonomous Region, China
    2Key Laboratory of Fertility Preservation and Maintenance of Ningxia Medical University, Ministry of Education, Key Laboratory of Reproduction and Heredity of Ningxia Hui Autonomous Region, Yinchuan 750004, Ningxia Hui Autonomous Region, China
  • Received:2012-01-05 Revised:2012-04-21 Online:2012-09-30 Published:2012-09-30
  • Contact: Wang Yan-rong, Professor, Department of Human Anatomy and Histology- Embryology, Ningxia Medical University Yinchuan 750004, Ningxia Hui Autonomous Region, China; Key Laboratory of Fertility Preservation and Maintenance of Ningxia Medical University, Ministry of Education, Key Laboratory of Reproduction and Heredity of Ningxia Hui Autonomous Region, Yinchuan 750004, Ningxia Hui Autonomous Region, China 4083304@163.com
  • About author:Cai Yu-fang★, Master, Associate professor, Department of Human Anatomy and Histology- Embryology, Ningxia Medical University Yinchuan 750004, Ningxia Hui Autonomous Region, China; Key Laboratory of Fertility Preservation and Maintenance of Ningxia Medical University, Ministry of Education, Key Laboratory of Reproduction and Heredity of Ningxia Hui Autonomous Region, Yinchuan 750004, Ningxia Hui Autonomous Region, China Cyfang_123@yahoo.com.cn

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Abstract:

BACKGROUND: The application of antioxidant plays a key role in improving the viability of frozen-thawed ovarian tissue.
OBJECTIVE: To explore the effects of lycium barbarum polysaccharides on fetal ovary tissue cryopreservation by observe the developmental condition of cryopreserved fetal ovarian tissue xenografts after transplanted into nude mice kidney capsule.
METHODS: The experiment was divided into four groups. ①Fresh transplanted group: the fresh fetal ovary tissue was transplanted directly. ②Cryopreservation control group: the cryoprotectant solution was the base fluid. ③β-mercaptoethanol group: the cryoprotectant solution was the basic fluid with 100 μmol/L β-mercaptoethanol. ④Lycium barbarum polysaccharides group: the cryoprotectant solution was the basic fluid with 400 μmol/L lycium barbarum polysaccharides. The ovarian cortex block was transplanted into the nude mice kidney capsule after freezing thawing, and the specimen was obtained at 12 weeks after transplantation.
RESULTS AND CONCLUSION: There was no significant difference of the survival rate in the grafts among groups (P > 0.05). The cryopreservation control group had the most follicle (P < 0.05). As for the survival rate of follicles, there was significant difference among groups; it was lowest in cryopreservation control group and highest in lycium barbarum polysaccharides group. Preservation of ovarian ultrastructure in β-mercaptoethanol and lycium barbarum polysaccharides group was better than that in the cryopreservation control group. Lycium barbarum polysaccharides with concentration of 400 mg/L and β-mercaptoethanol with concentration of 100 mg/L is benefit to the ovarian tissue cryopreservation and can significantly improve the survival ratio of cryopreserved ovarian tissue after transplantation.

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