Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (40): 7470-7474.doi: 10.3969/j.issn.2095-4344.2012.40.012

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Effect of frozen carriers on the outcomes of blastocysts freezing-thawing in the expanding period

Gong Xiao-yun, Long Mei, Hu Bo, Zhao Jing, Wang Peng, Li Xia   

  1. Reproductive Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011 , Xinjiang Uygur Autonomous Region, China
  • Received:2012-06-04 Revised:2012-07-09 Online:2012-09-30 Published:2012-09-30
  • Contact: Li Xia, Attending physician, Lecturer, Reproductive Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011 , Xinjiang Uygur Autonomous Region, China Lx_shz@126. com
  • About author:Gong Xiao-yun★, Master, Attending physician, Lecturer, Reproductive Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011 , Xinjiang Uygur Autonomous Region, China 18999820257@189.cn

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Abstract:

BACKGROUND: Blastocyst freezing-thawing has great significance to the development of the human assisted reproductive technology; favorable frozen carrier can reduce the frozen toxic and improve refrigeration efficiency, which has been considered as the hot spot in improving the outcome of blastocyst freezing-thawing.
OBJECTIVE: To discuss the effects of different frozen carriers on the outcomes of Kunming mice blastocyst vitrification freezing-thawing in expanding period.
METHODS: The fresh Kunming mouse blastocysts in the expanding period were considered as the control group, and then the mouse embryo were randomly selected from the same period for the vitrification thawing, and before frozen, artificial shrinkage was performed by the laser line; the vitrification method was used for freezing and thawing, and the embryos were divided into cryoloop group, closed pulled straw group and self-made straw-leaf group according to different experiment carriers. The duration of contact cryoprotectant before input liquid nitrogen were < 40 seconds, 40-60 seconds and 60-90 seconds in cryoloop group and self-made straw-leaf group; after thawing, 10% serum replacement was added in to the blastocyst medium supplemented for the micro-drop culture in embryonic sac period. The effects of the vitrified mouse blastocysts were assessed by the lost rate, recovery rate and hatched rate after cultured for 24 hours.
RESULTS AND CONCLUSION: Compared with closed pulled straw group, the lost rate of the blastocysts was reduced and the recovery rate of the embryo was increased in cryoloop group and self-made straw-leaf group (P < 0.05), while the hatched rate after cultured for 24 hours in the freezing groups was lower than that in the control group (P < 0.05). There was no significant difference of the recovery rate and hatched rate in cryoloop group and self-made straw-leaf group when the duration of contact cryoprotectant before input liquid nitrogen was 90 seconds. These findings indicate that cryoloop carrier and self-made straw-leaf carrier has a good effect on blastocyst vitrification freezing-thawing in the expanding period.

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